Safety assessment of mammary gland stem cells from normal tissues in breast cancer patients

doi:10.3969/j.issn.2095-4344.2016.41.007

Abstract

Abstract:

BACKGROUND: The cultivation of mammary gland stem cells is of great significance for the development of mammary gland and breast cancer.
OBJECTIVE: To seek an easy method to isolate and culture mammary gland stem cell in vitro, and verify the safety of cells.
METHODS: Mammary epithelial cells were isolated from normal tissues surrounding breast cancer, and CD49f- and EPCAM-positive cells were sorted using flow cytometry followed by surface marker analysis and cell colony formation ability analysis. Afterwards, real-time fluorescent quantitative PCR was used to detect C-erbB-2 and Maspin mRNA expression in mammary gland stem cells, breast cancer tissues and normal tissues surrounding breast cancer.
RESULTS AND CONCLUSION: Human mammary gland stem cells were successfully cultured and highly expressed CD49f and EPCAM, with the presence of mixed colony, pleural epithelial cell colony, and myoepithelial cell colony. c-erbB-2 was lowly expressed while Maspin highly expressed in mammary gland stem cells. Our experimental findings indicate that the mammary gland stem cells derived from normal tissue surrounding breast cancer have biological safety.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Breast Neoplasms, Neoplastic Stem Cells, Genes, erbB-2, Tissue Engineering

CLC Number:

R394.2

Bi Xiao-juan, Fu Ming-gang, Guo Li-ying, Liu Sha, Dilimina Yilamu, Guo Chen-ming . Safety assessment of mammary gland stem cells from normal tissues in breast cancer patients[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6125-6130.

Figures/Tables 1

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