Correlation of matrix metalloproteinase 2 and 9 with the early performance of knee osteoarthritis cartilage under MRI

doi:10.3969/j.issn.2095-4344.2016.37.007

Abstract

Abstract:

BACKGROUND: Matrix metalloproteinases (MMPs) have been shown to break the balance between the production and degradation of extracellular matrix by degrading collagen, gelatin, elastin and other macromolecules, which is the leading cause of bone and cartilage damage in rheumatic arthritis.
OBJECTIVE: To determine the expression levels of MMP-2 and MM-9 in the rabbit knee osteoarthritis cartilage, and to analyze whether they are related to the early MRI performance.
METHODS: Fifty healthy Japanese white rabbits free from osteoarthritis were enrolled and randomly divided into control group (n=10) and model group (n=40). The anterior and posterior cruciate ligament amputation was adopted to establish the model of osteoarthritis. Subsequently, MRI examination and the expression levels of MMP-2 and MMP-9 were detected at 1, 3, 5 and 7 weeks (n=10 at each time point), respectively.
RESULTS AND CONCLUSION: MRI examination showed that more damaged cartilage appeared with time in the rabbit model of osteoarthritis, and significantly different from MRI classification (P < 0.05). The expression levels of MMP-2 and MMP-9 mRNA in the model group were always significantly higher than those in the control group (P < 0.05); MMP-2 level reached the highest at 3 weeks after modeling (P < 0.01), and then presented a slight decline; MMP-9 level peaked at 5 weeks after modeling (P < 0.01) and slightly decreased at 7 weeks. MRI classification of cartage injury and expressions of MMP-2 (R2=0.119, P=0.119) and MMP-9 (R2= 0.466, P=0.466) were shown to have the correlation. These results illustrate that the serum levels of MMP-2 and MMP-9 are related to the early destruction of articular cartilage in patients with osteoarthritis, and can be used as predictors for early osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Magnetic Resonance Imaging, Tissue Engineering

CLC Number:

R318

Li Guang, Guo Qi-fa, Huang Ning-qing, Li Ling-wei. Correlation of matrix metalloproteinase 2 and 9 with the early performance of knee osteoarthritis cartilage under MRI[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(37): 5518-5523.

Figures/Tables 1

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