Biological properties of nanohydroxyapatite composite for dental restoration

doi:10.3969/j.issn.2095-4344.2016.34.005

Abstract

Abstract:

BACKGROUND: Nanohydroxyapatite composition is similar to that of the natural bone, and because of its characteristics of nanomaterials, it has been widely used in the bone defect repair.
OBJECTIVE: To observe the effect of nanohydroxyapatite composite materials in the repair of alveolar bone defect in animal models by detecting its cytotoxicity.
METHODS: (1) In vitro experiment: nanohydroxyapatite extracts with concentrations of 10%, 50%, 100% (or hydroxyapatite leaching extracts) were used to culture periodontal ligament fibroblast-like cells and L-929 cells for 7 days, respectively, followed by cell cytotoxicity analysis. (2) In vivo experiment: 45 adult rabbits were used to make animal models of alveolar bone defects and then were randomized into three groups: experimental group implanted with nanohydroxyapatite, control group with hydroxyapatite, and blank control group with no implant material. New bone formation was observed at 1, 2 and 3 weeks after operation.
RESULTS AND CONCLUSION: (1) In vitro experiment: after directly cultured with 10%, 50%, 100% nanohydroxyapatite extracts, the cell cytotoxicity was graded 0 to 1, indicating no presence of cytotoxicity. (2) In vivo experiment: in the three groups, the percentage of new bone area accounting for the total visual field was gradually in a rise over time. Moreover, the amount of new bone tissues was higher in the experimental group than the other two groups at different time (P < 0.05). These findings demonstrate that the nanohydroxyapatite materials have no cytotoxicity that can be used to promote the repair of alveolar bone defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Nanostructures, Hydroxyapatites, Tissue Engineering

CLC Number:

R318

Huang Tao, Chen Han. Biological properties of nanohydroxyapatite composite for dental restoration[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(34): 5045-5050.

Figures/Tables 4

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