Epimedium combined with bone marrow mesenchymal stem cell transplantation for treatment of kidney disease in rats

doi:10.3969/j.issn.2095-4344.2016.32.011

Abstract

Abstract:

BACKGROUND: A large number of studies have shown that bone marrow mesenchymal stem cells (BMSCs) with differentiation ability can be used to treat kidney injury. Epimedium has good anti-inflammatory and immune restoration ability.
OBJECTIVE: To observe the effect of epimedium with BMSC transplantation on rat’s kidney disease caused by adriamycin nephrosis.
METHODS: Fifty rats were equivalently randomized to receive no treatment in control group, tail vein injection of normal saline plus intragastric injection of normal saline in model group, tail vein injection of BMSCs plus intragastric injection of epimedium decoction in combined group, tail vein injection of BMSCs in BMSCs group, and intragastric injection of epimedium decoction in epimedium group. Rat models of adriamycin nephrosis were made in the latter four groups through tail vein injection of doxorubicin hydrochloride, while rats in the control group were only given the same volume of normal saline. Twenty-four hours after modeling, rats in the latter four groups began to be given the corresponding treatments. Epimedium decoction was given in the combined and epimedium groups at a dose of 1 mL/d, for consecutive 1 week.
RESULTS AND CONCLUSION: Compared with the model group, mitigated kidney injury and reduced urinary protein level were found in rats undergoing BMSCs and/or epimedium decoction treatment; the levels of plasma albumin and catalase were significantly increased in the combined group and epimedium group, while the levels of serum cholesterol, triglycerides and malondialdehyde decreased; the mRNA expression of transforming growth factor β1 in the kidney tissues was dramatically declined in the combined group and BMSC group. To conclude, the combined use of epimedium and BMSCs is superior to their use alone in the treatment of kidney disease.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Epimedium, Nephrosis;, Tissue Engineering

CLC Number:

R394.2

Chen Xiao-yong, Wang Zi-chuang, Zhang Jian-wei, Han Jia-rui. Epimedium combined with bone marrow mesenchymal stem cell transplantation for treatment of kidney disease in rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(32): 4785-4791.

Figures/Tables 4

2.1 骨髓间充质干细胞形态原代骨髓间充质干细胞呈圆形或梭形(图1A)。第2代骨髓间充质干细胞多为梭形(图1B)。第5代骨髓间充质干细胞成均匀长梭形状态，边缘清晰可见(图1C)。实验采用第5代骨髓间充质干细胞进行治疗。 2.2 实验动物数量分析实验中所用到的SD大鼠50只，全部进入结果分析，无脱失。 2.3 24 h尿蛋白含量与对照组相比，模型组大鼠24 h尿蛋白含量明显升高(P < 0.01)；与模型组相比，骨髓间充质干细胞+淫羊藿组大鼠24 h尿蛋白含量显著降低(P < 0.01；表1)。 2.4 血生化水平与对照组相比，模型组大鼠血清肌酐水平显著升高(P < 0.05)；与模型组相比，骨髓间充质干细胞+淫羊藿组和骨髓间充质干细胞组大鼠血清肌酐水平显著减低(P < 0.05)。各组大鼠血清尿素氮和内生肌酐清除率差异无显著性意义(P > 0.05；表2)。与对照组相比，模型组大鼠血清蛋白含量显著下降(P < 0.05)，血清胆固醇和三酰甘油含量显著升高(P < 0.05)；与模型组相比，骨髓间充质干细胞+淫羊藿组和淫羊藿组大鼠血浆白蛋白显著升高(P < 0.05)，血清胆固醇和三酰甘油浓度显著降低(P < 0.05；表3)。与对照组相比，模型组大鼠血液中过氧化氢酶水平显著下降，丙二醛浓度显著上升(P < 0.05)；与模型组相比，骨髓间充质干细胞+淫羊藿组和淫羊藿组大鼠血液中过氧化氢酶含量显著升高，丙二醛浓度显著降低 (P < 0.05；表4)。 2.5 肾组织病理形态变化对照组大鼠肾脏无异常变化(图2A)；模型组大鼠肾脏组织中可见球囊型粘连，肾小球肥大肿胀，毛细血管腔变窄，有空泡变形，蛋白管型增多(图2B)；给予淫羊藿和/或骨髓间充质干细胞治疗后，大鼠肾脏组织中肾小管上皮细胞的肿胀及空泡明显减少，蛋白管型减少(图2C-E)。对照组大鼠肾脏组织中肾小球足细胞正常，肾小球基底膜完整均匀整齐(图3A)，模型组大鼠肾脏组织中足细胞水肿，足细胞广泛融合在一起，淋巴细胞浸润，基底膜增厚(图3B)；给予淫羊藿和/或骨髓间充质干细胞后，大鼠肾脏组织中足细胞消肿，融合程度有所改善，基底膜变薄，无淋巴细胞浸润(图3C-E)。 2.6 肾脏组织中转化生长因子β1 mRNA的表达水平与模型组相比，骨髓间充质干细胞+淫羊藿组和骨髓间充质干细胞组大鼠肾脏组织中转化生长因子β1 mRNA表达显著下降(P < 0.05；表5)。"

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