Biological properties of acellular dermal matrix derived from mature scar tissue versus normal skin

doi:10.3969/j.issn.2095-4344.2016.30.013

Abstract

Abstract:

BACKGROUND: It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study.
OBJECTIVE: To compare the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix of mature scar tissue and normal skin.
METHODS: The acellular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acellular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cell growth curve was drawn. Additionally, the acellular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation.
RESULTS AND CONCLUSION: There were many gaps but no cellular components in the acellular dermal matrix, in both two groups. Collagen fibers of the acellular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly; the biomechanical properties of the acellular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acellular dermal matrix, and the growth curve was basically consistent. After 4 weeks of implantation, more inflammatory cells infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cells infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, collagen fibers arranged in neat, and small vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Cicatrix, Skin, Biomechanics, Tissue Engineering

CLC Number:

R318

Wang Yang-yang, Li Xiao-jing, Bu Shou-shan. Biological properties of acellular dermal matrix derived from mature scar tissue versus normal skin[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(30): 4496-4502.

Figures/Tables 6

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