Effects of static pressure on the cytocompatibility of adipose-derived stem cells on electrospun nanofibers

doi:10.3969/j.issn.2095-4344.2016.30.008

Abstract

Abstract:

BACKGROUND: Electrospun polylactic acid/polycaprolactone nanofibers (ENF) are a kind of self-synthesized biodegradable material. Our preliminary studies have indicated that the biomaterial exhibits excellent biocompatibility; however, the research about its mechanics is still little.
OBJECTIVE: To explore the effects of static pressure on the cytocompatibility of adipose-derived stem cells on the ENF scaffold.
METHODS: Adipose-derived stem cells were seeded onto the ENF scaffold, and then cultured in the low-glucose DMEM supplemented with 10% fetal bovine serum. The mixed constructs were submitted to the static pressure at 0, 15, 30, and 45 kPa for 4 hours using a static pressure device, respectively. Subsequently, the proliferation, adhesion and viability of adipose-derived stem cells on the ENF scaffold were detected using MTT assay and living/dead staining to evaluate the cytocompatibility.
RESULTS AND CONCLUSION: MTT assay showed that there were significant differences in absorbance values among groups by one-way analysis of variance after 4 hours of loading with different static pressures in vitro. Under 0-30 kPa static pressure, the absorbance values increased with static pressure, but the absorbance values declined until the pressure reached 45 kPa, and multiple comparisons between groups showed significant difference. The significant differences in the cell attachment percentage by MTT assay could be found among groups. The living/dead staining results supported the above findings. Furthermore, the significant differences in percentage of living cells among groups were shown using either one-way analysis of variance or paired t test. In conclusion, the appropriate static pressure can promote the cytocompatibility, proliferation, adhesion and viability of adipose-derived stem cells on the ENF scaffold. But the excessive pressure is likely to inhibit the cellular biological behaviors, thus affecting cytocompatibility of adipose-derived stem cells with the ENF scaffold.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Adipose Tissue, Stem Cells, Nanofibers, Pressure, Tissue Engineering

CLC Number:

R318

Chen Liang, Bai Yi, Liu Kai, Zhang Wei. Effects of static pressure on the cytocompatibility of adipose-derived stem cells on electrospun nanofibers[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(30): 4463-4468.

Figures/Tables 4

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