Preparation of an acellular porcine corneal stroma scaffold and its biocompatibility

doi:10.3969/j.issn.2095-4344.2016.30.006

Abstract

Abstract:

BACKGROUND: Studies have found that a variety of biological materials can be used for preparing corneal stroma scaffolds that have good biocompatibility, but research on preparation and biocompatibility of the acellular porcine corneal stroma scaffold is little.
OBJECTIVE: To explore the preparation and biocompatibility of the acellular porcine corneal stroma scaffold.
METHODS: Acellular porcine corneal stroma scaffold and its extract were prepared. Well-grown human corneal stromal cells were selected and cultured in the extract of acellular porcine corneal stroma scaffold (experimental group) or in the complete medium (control group), respectively. After 1, 2 and 3 days of culture, the proliferation ability of human corneal stromal cells was detected by MTT assay. In the meanwhile, human corneal cells were directly seeded onto the acellular porcine corneal stroma scaffold, and then the cell growth on the scaffold was detected using immunochemical method.
RESULTS AND CONCLUSION: The number of human corneal stromal cells was in a rise with time in the two groups, and absorbance values had no significant difference between two groups at different time points of culture. Human corneal stromal cells grew well on the scaffold, and were positive for cell integrin β1, vimentin, aldehyde dehydrogenase 3A1, as well as CD34, CDK2 and K-Ras. These results show that the acellular porcine corneal stroma scaffold has no cytotoxicity, and has good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Corneal Stroma, Tissue Engineering

CLC Number:

R318

Huo Yin-ping, Zhou Li-xiao, Sun Cheng-lin. Preparation of an acellular porcine corneal stroma scaffold and its biocompatibility[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(30): 4449-4455.

Figures/Tables 5

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