Characterization of UGT1a1, UGT1a6 and mGST1 at different stages of differentiation of mouse embryonic stem cells

doi:10.3969/j.issn.2095-4344.2016.28.004

Abstract

Abstract:

BACKGROUND: Until now, limited information has been neported on the characterization of udines 5-diphosphateglucuronosyl transferase (UGT) 1a1, UGT1a6, and microsomal glutathione S-transferase 1 (mGST1) during the differentiation of mouse embryonic stem cells.
OBJECTIVE: To observe the expression characteristics of UGT1a1, UGT1a6 and mGST1 at different stages of differentiation of mouse embryonic stem cells.
METHODS: Embryonic fibroblasts from Wistar rats were isolated and cultured as feeder cells. Mouse embryonic stem cells seeded onto these feeder cells were induced to differentiate into hepatocytes. Subsequently, expression of UGT1a1, UGT1a6 and mGST1 was detected using western blot assay, and catalytic activity of mGST1 was determined by high performance liquid chromatography.
RESULTS AND CONCLUSION: Increasing UGT1a1 expression was visible during the whole cell differentiation, while UGT1a6 exhibited no expression initially but a higher level at 18 days of differentiation. mGST1 expression was visible at high level throughout the differentiation, but its expressive abundance was still lower than that in the adult mouse stem cells. At 18 days after the beginning of differentiation, the catalytic activity of mGST1 in microsomal liver tissue was 7.65 μmol/(min•g). Taken together, stabilized UGT1a1, increased UGT1a6 and little mGST1 expression are confirmed in the differentiation of embryonic stem cells into hepatocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Embryonic Stem Cells, Hepatocytes, Tissue Engineering

CLC Number:

R394.2

Xu Ling-li. Characterization of UGT1a1, UGT1a6 and mGST1 at different stages of differentiation of mouse embryonic stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4130-4135.

Figures/Tables 2

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