Tissue-engineered acellular dermis repairs a skin ulcer: its preparation and healing mechanism

doi:10.3969/j.issn.2095-4344.2016.25.014

Abstract

Abstract:

BACKGROUND: In recent years, acellular human cadaveric dermis has been applied for repairing dermal defects and filling soft tissues, but this repair material is expensive and difficult to obtain.

OBJECTIVE: To explore the repair effect of tissue-engineered acellular dermis on wound healing of skin ulcer.

METHODS: Sprague-Dawley rats were randomly divided into autologous reticular dermis and 2.5 g/L trypsin, 0.5% Triton X-100 and PBS solution, and in the meanwhile, a rat model of skin ulcer was established. Then, autologous skin graft and tissue-engineered acellular dermis were transplanted into the autologous reticular dermis group and acellular dermal group, respectively. Subsequently, comparative study about the repair effect and relative mechanism between two groups was performed.

RESULTS AND CONCLUSION: The tissue-engineered acellular dermis showed a white and grainy shape at room temperature. And hematoxylin-eosin staining showed that: the internal structure of tissue- engineered acellular dermis exhibited a dendritic distribution, and the gap between collagen fiber bundles was relatively large. At 2, 3, 4 and 8 weeks after dermal transplantation, the survival rate of skin graft in the acellular dermis group was significantly higher than that in the autologous reticular dermis group (P < 0.05). And the wound contraction rate in the acellular dermis group was significantly higher than that of the autologous reticular dermis group at 3, 4 and 8 weeks after dermal transplantation (P < 0.05). In addition, the average diameter and clearance rate of collagen fibers in the normal skin around wound in the acellular dermis group were significantly higher than those of autologous reticular dermis group (P < 0.05). To conclude, it is relatively simple to prepare tissue-engineered acellular dermis under mild conditions, and the prepared tissue-engineered acellular dermis presents a regular shape. Moreover, this acellular dermis achieves desired outcomes in repairing dermal defects, which can promote wound healing by reducing the intradermal DNA residual and significantly improving some biomechanical properties in vivo.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Burns, Skin, Ulcer, Tissue Engineering

CLC Number:

R318

Du Peng, Guan Zhen, Wang Xiao-chuan. Tissue-engineered acellular dermis repairs a skin ulcer: its preparation and healing mechanism[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3738-3743.

Figures/Tables 7

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