Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (24): 3562-3567.doi: 10.3969/j.issn.2095-4344.2016.24.010

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Effects of pre-vascularization of tissue-engineered constructs on the survival of transplanted pancreatic islets

Zhao Gao-ping1, 2, Deng Shao-ping1, Huang Xiao-lun2, Yang Mao-zhu2, Wei Ling-ling2, Li Shu-rong2, Wei liang2   

  1. 1Department of Gastrointestinal Surgery, Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital, the Affiliated Hospital of University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China; 2Sichuan Provincial Key Laboratory for Translational Medicine of Organ Transplantation, Chengdu 610072, Sichuan Province, China
  • Online:2016-06-10 Published:2016-06-10
  • Contact: Deng Shao-ping, M.D., Professor, Department of Gastrointestinal Surgery, Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital, the Affiliated Hospital of University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China
  • About author:Zhao Gao-ping, M.D., Associate chief physician, Department of Gastrointestinal Surgery, Sichuan Academy of Medical Science & Sichuan Provincial People’s Hospital, the Affiliated Hospital of University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, China; Sichuan Provincial Key Laboratory for Translational Medicine of Organ Transplantation, Chengdu 610072, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81172832; Sichuan Youth Science and Technology Foundation, No. 2013JQ0020; Special Program for Sichuan Youth Science and Technology Innovation, No. 2014TD0010; a grant from Health and Family Planning Commission of Sichuan Province. No. 110190

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Abstract:

BACKGROUND: Pancreatic islet transplantation via portal vein system leads to the apoptsis of a number of islet cells due to local hypoxia, thereby affecting transplant outcomes.
OBJECTIVE: To explore the effect of pre-micrvascularization network of tissue-engineered constructs on the survival of transplanted islets and the feasibility of xenogenic islet transplantation.
METHODS: A 5-mm-long cylindrical silicone tube filled with Matrigel TM matrix surrounding the superficial epigastric vessel was placed in the groin of diabetic mice. After the syngeneic islets with 300 islet equivalents (IEQ) were transplanted into the silicone chamber on days 0, 14 and 28 post-chamber implantation, respectively, the recovery time of blood glucose was observed. The islets with the quantity of 100 IEQ, 200 IEQ and 300 IEQ, respectively, were transplanted on day 28 post-implantation and then the blood glucose level was determined. Moreover, the survival of human pancreatic islets with 1 000 IEQ transplanted into the pre-vascularizated chamber or under the renal capsule of diabetic mice, followed by the treatment of anti-CD45RB and/or anti-CD40L (MR-1) was analyzed.
RESULTS AND CONCLUSION: An abundant micro-vascularized network was established in the silicone chamber on day 28 post-implantation. The time of the blood glucose returning to normal level in diabetic mice was negatively correlated with the time required for pre-vascularization and the number of implanted islets. No islet grafts implanted in the silicone chamber and treated by anti-CD45RB survived for long term. However, one of seven (14.3%) grafts survived for long term, which was not significantly different from the transplantation under the renal capsule group (n=8, MST > 71 days, P > 0.05). The tissue-engineered pre-vascularization network markedly extends the survival time of the islet grafts before transplantation. The transplantation of the xenogenic pancreatic islets into the vascularized silicone chamber might be a promising method in the future clinical application.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Diabetes Mellitus, Islets of Langerhans Transplantation, Survival Rate

CLC Number: