Effect of zein/chitosan composite membrane on the osteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2016.21.005

Abstract

Abstract:

BACKGROUND: Some scholars have prepared zein/chitosan composite membrane based on blending methods, and preliminary evaluation of its physical and chemical properties shows that chitosan partly improves the mechanical properties and hydrophilic properties of zein. Therefore, zein/chitosan composite membrane presumably has good cytocompatibility, which is beneficial to osteogenic differentiation of bone marrow mesenchymal stem cells.

OBJECTIVE: To explore the effect of zein/chitosan composite membrane on the differentiation of bone marrow mesenchymal stem cells into osteoblasts and its feasibility as a bone tissue-engineered material.

METHODS:With 60% acetic acid as solvent, zein/chitosan composite membrane was prepared by blending and casting method. The structure and physicochemical properties of the composite membrane were investigated by Fourier transform infrared spectroscopy, tensile testing, water absorption testing and scanning electron microscopy. And the cytocompatibility of the membrane was evaluated by in vitro cell cufture. Besides, bone marrow mesenchymal stem cells from Sprague-Dawley rat were isolated via adherence screening method, and the effects of the composite membrane on the osteogenic differentiation of these cells were observed by scanning electron microscopy, fluorescent labeling and alkaline phosphatase assay.

RESULTS AND CONCLUSION: The tensile strength, water absorption and hydrophilicity of the films were improved with the chitosan increased; chitosan could promote cell proliferation indicating the good cytocompatibility of the composite films. Moreover, osteogenic induction occurred in bone marrow mesenchymal stem cells cultured on the composite membrane, and with an increase of chitosan, the induction was promoted. In conclusion, zein/chitosan composite membrane can be applied widely in the field of bone tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Zein, Chitosan, Biocompatible Materials, Tissue Engineering

CLC Number:

R318

Li Chun-liang, Qin Feng, Li Lin-chang, Tang Bao-ming, Li Zhao-wei. Effect of zein/chitosan composite membrane on the osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3071-3079.

Figures/Tables 11

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