Propofol pretreatment combined with umbilical blood mesenchymal stem cell transplantation improves cerebral ischemia-reperfusion injuries

doi:10.3969/j.issn.2095-4344.2016.19.011

Abstract

Abstract:

BACKGROUND: As propofol has a neuroprotective effect, and umbilical cord blood mesenchymal stem cells have a high differentiation potential, their combination will have a better therapeutic effect on cerebral ischemia-reperfusion injury.
OBJECTIVE: To study the effect of propofol pretreatment combined with umbilical blood mesenchymal stem cell transplantation on cerebral ischemia-reperfusion injury in rats.
METHODS: Sixty-three Sprague-Dawley rats were randomized into model, propofol, and combined group (n=21 per group). Rat models of cerebral ischemia-reperfusion injury were made using ligation of the middle cerebral artery occlusion in the three groups. Rats in the combined group were given 100 mg/kg propofol injection at 1 day before injury and injection of umbilical blood mesenchymal stem cells via the tail vein (0.5 mL, 2×10⁹/L).
RESULTS AND CONCLUSION: Compared with the model group, the neurological function was improved significantly in the propofol and combined group, especially in the latter one, presenting with a remarkable mitigation in brain injury and an increased level of survivn mRNA in the rat hippocampus. The content of serum malondialdehyde was lower but the activity of superoxide dismutase was higher in the combined group compared with the propofol group. These findings indicate that propofol pretreatment combined with umbilical blood mesenchymal stem cell transplantation has better therapeutic effects than propofol pretreamtnet alone for improving cerebral ischemia-reperfusion injury in rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cord Blood Stem Cell Transplantation, Propofol, Reperfusion Injury, Tissue Engineering

CLC Number:

R394.2

Hou Shao-ke, Hao Li-na, Wei Jiao, Zhou Ya-jing . Propofol pretreatment combined with umbilical blood mesenchymal stem cell transplantation improves cerebral ischemia-reperfusion injuries[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(19): 2810-2816.

Figures/Tables 3

2.1 脐血间充质干细胞的形态原代培养的脐血间充质干细胞呈圆形及不规则形；培养5 d左右时，出现贴壁细胞，逐渐伸出突起，有胞体较大、多核的破骨样细胞出现；培养八九天时，可见呈漩涡状生长的集落细胞，铺满瓶底，部分呈长梭形或多角形；培养10-14 d时，细胞数量增加，呈集落状。随着脐血间充质干细胞传代次数的增加，细胞集落进一步扩大，破骨样细胞逐渐减少消失，呈梭形的成纤维样细胞均匀分布，传代脐血间充质干细胞可达80%融合(图1)。 2.2 实验动物数量分析 3组大鼠均进入结果分析，无脱失。 2.3 神经功能评分造模后1 d各组大鼠神经功能评分接近(P > 0.05)；造模后3，7，14，21 d联合组和异丙酚组大鼠神经功能评分明显低于模型组(P < 0.05)，且联合组大鼠神经功能评分低于异丙酚组(P < 0.05；表1，图2)。 2.4 脑组织病理形态苏木精-伊红染色结果显示，模型组大鼠缺血损伤中心区存在大量神经元胞核破裂、溶解、水肿等细胞坏死现象，细胞形态由多极形变为圆形。经异丙酚预处理后大鼠缺血损伤中心区神经元凋亡、坏死减少；而其联合脐血间充质干细胞干预后可见凋亡、坏死的神经元明显减少，神经元结构完整(图3)。 2.5 survive基因表达水平反转录PCR检测结果显示，脑缺血再灌注损伤大鼠经异丙酚处理后，survivn基因的表达水平明显增加(P < 0.05)，且异丙酚预处理联合脐血间充质干细胞移植对survivn基因表达水平的提升效果优于单纯异丙酚预处理(P < 0.05；表2，图4)。 2.6 血清丙二醛和超氧化物歧化酶的含量与异丙酚组相比，联合组大鼠血清中丙二醛含量较低，而超氧化物歧化酶含量较高(P < 0.05；表3，图5)。"

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