Mandibular defects repaired by coral bone with platelet rich plasma

doi:10.3969/j.issn.2095-4344.2016.16.011

Abstract

Abstract:

BACKGROUND: Platelet rich plasma contains various growth factors, such as platelet-derived growth factor, metastatic growth factor, insulin-like growth factor, epidermal growth factor as well as vascular endothelial growth factor. Therefore, it can directly or indirectly promote cell differentiation and proliferation in different stages of bone regeneration.

OBJECTIVE: To study the effects of coral bone with platelet rich plasma in the repair of mandibular defects.

METHODS: Totally 24 New Zealand white rabbits were randomly divided into three groups (n=8 per group) including test group, control group and blank control group. Coral bone with autologous platelet rich plasma, coral bone or nothing was implanted, respectively, after establishing unilateral mandibular defect models. The defects were evaluated by imaging observation and bone his-tomorphometric analysis at 2, 4, 8, 12 weeks after surgery.

RESULTS AND CONCLUSION: At 12 weeks after surgery, by imaging observation, density of the defect increased in the blank control group, which was lower than that of the normal bone; the bone density in the test group was higher than that in the control group, both of which were similar with the normal bone. Besides, the materials were closely combined with the new tissues. By bone his-tomorphometric analysis, area of the new bone in the test group was significantly larger than that in the control and blank control group (P < 0.05). In conclusion, coral bone with platelet rich plasma has good biocompatibility and bone conductivity, which can induce bone regeneration to promote defect repair.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Platelet-Rich Plasma, Mandible, Tissue Engineering

Jiang Liu-hong, Dong Ying, Yan Chun-ge, Liu Yan-hui, Jing Xiang-dong. Mandibular defects repaired by coral bone with platelet rich plasma[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2360-2368.

Figures/Tables 7

References

[1] Nilsson ES,Urist MR,Dawson EG,et al.Bone repair induced by bone morphogenetic protein in ulnar defects in dogs.J Bone Joint Surg. 1986;68B: 635-642.

[2] Burchardt H.Related Articles,The biology of bone graft repair.Clin Orthop. 1983;(174):28-42.

[3] Enneking WF,Eady JL,Burchardt H.Autogenous cortical bone grafts in the reconstruction of segmental skeletal defects.J Bone Joint Surg Am. 1980;62(7): 1039-1058.

[4] Pazzaglia UE.Periosteal and endosteal reaction to reaming and nailing: the possible role of revascularization on the endosteal anchorage of cementless stems. Biomaterials. 2012;17(10):1009-1014.

[5] Weng D,Hürzeler MB,Quiñones CRet al.Contribution of the periosteum to bone formation in guided bone regeneration. A study in monkeys.Clin Oral Implants Res.2014;11(6):546-554.

[6] Aitasalo K,Lehtinen R.The influence of a free periosteal transplant on bone healing in the irradiated tibia. A radiological histological and biochemical study on rabbits. Scand J Plast Reconstr Surg. 1985;19(3): 237-244.

[7] Zhang C,Hu YY,Cui FZ,et al.A study on a tissue- engineered bone using rhBMP - 2 induced periosteal cells with a porous nano -hydroxyapatite/collagen/poly (L-lactic acid) scaffold.Biomed Mater.2006;1(2):56-62.

[8] Schmidt BL,Kung L,Jones C,et al.Induced osteogenesis by periosteal distraction. J Oral Maxillofac Surg.2002;60(10):1170-1175.

[9] Sencimen M,Aydintug YS,Ortakoglu K,et al. Histomorphometrical analysis of new bone obtained by distraction osteogenesis and osteogenesis by periosteal distraction in rabbits.Int J Oral Maxillofac Surg.2007;36(3):235-242.

[10] Glowacki J,Shusterman EM,Troulis M,et al.Distraction osteogenesis of the porcine mandible: histomorphometric evaluation of bone.Plast Reconstr Surg.2004;113(2):566-573.

[11] 曾融生,任材年,于秦曦.珊瑚人工骨作为颌面骨修复材料的初步报告[J].中华口腔医学杂志, 1991,26(6):345-347.

[12] Souyris F, Pellequer C.Coral,a new biomedical material: experimental and first clinical investigation on Madreporaria.J Maxillfac Surg.1985;13:64-69.

[13] 郑有华,任材年,张志光,等.珊瑚人工骨/自体骨髓复合移植修复颌骨缺损临床应用[J].中国现代医学杂志, 2005, 15(8):1227-1230

[14] 郑有华,任材年,陈小华,复合珊瑚骨修复颌骨实验性骨缺损[J].临床口腔医学杂志,1996,12(3):143-145.

[15] Chen F,Mao T,Tao K,et al.Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model.J Oral Maxillofac Surg. 2002;60:1155-1159.

[16] Guillemin G,Patat JL.The use of coral as a bone graft substitutes.J Biomed Mater Res.1987;21:557-567.

[17] Weibrich G,Kleis WK,Hafner G.Growth factor levels in the platelet-rich plasma produced by 2 different methods:curasan-type PRP Kit versus PCCS PRP system.Int J Oral Maxillofac Implants. 2002;17(2): 184-190.

[18] Cenni E,Granchi D,Vancini M,et al.Platelet release of transforming growth factor-β and β-thromboglobulin after in vitro contract with acrylic bone cements. Biomaterials.2002;23(6):1479-1484.

[19] Casap N,Venezia NB,Wilensky A,et al.VEGF facilitates periosteal distraction- induced osteogenesis in rabbits: a micro-computerized tomography study. Tissue Eng Part A.2008;14(2):247-253.

[20] Gruber R,Mayer C,Bobacz K,et al.Effects of cartilage-derived morphogenetic proteins and osteogenic protein-1 on osteochondrogenic differentiation of periosteum-derived cells. Endocrinology. 2014;142(5):2087-2094.

[21] Nakahara H,Goldberg VM,Caplan AI. Cultured- expanded periosteal-derived cells exibit osteochondrogenic potential in porous calcium phosphate ceramics in vivo.Clin Orthop Relat Res. 2013;(276):291-298.

[22] Breitbart AS,Grande DA,Kessler R,et al.Tissue engineered bone repair of calvarial defects using cultured periosteal cells.Plast Reconstr Surgy.2014 Mar;101(3):567-74.

[23] Sakata Y,Ueno T,Kagawa T,et al.Osteogenic potential of cultured human periosteum-derived cells - a pilot study of human cell transplantation into a rat calvarial defect model.J Craniomaxillofac Surg. 2014;34(8): 461-465.

[24] Zhu SJ,Choi BH,Huh JY,et al.A Comparative qualitative histological analysis of tissue-engineered bone using bone marrow messenchmal stem cells,alveolar bone cells and periosteal cells.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2013; 101(2):164-169.

[25] Mizuno H,Hata K,Kojima K,et al.A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum.Tissue Eng. 2015;12(5):1227-1335.

[26] Schilephake H.Bone growth factors in maxillofacial skeletal reconstruction.Int J Oral Maxillofac Surg. 2002; 31(5):469-484.

[27] 陈鹏,刘冰.活性纳米羟基磷灰石复合胶原/聚乳酸材料修复颅骨极限缺损的实验研究[J].中国修复重建外科杂志, 2007,21(11):1191-1195.

[28] Karageorgiou V,Kaplan D.Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2015;26(27): 5474-5479.

[29] Gauthier O,Bouler JM,Aguado E,et al.Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials.1998;19(1-3):133-139.

[30] 马红梅,吴琳,艾红军,等. 纳米羟基磷灰石/胶原/聚乳酸支架材料对人成骨细胞早期附着影响的实验研究[J].生物医学工程与临床,2007,11(3):161-163.

[31] Weibrich G,Hansen T,Kleis W, et al.Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration.Bone.2014;34(4):665-671.

[32] Kim SG,Chung CH,Kim YK,et al.Use of particulate dentin-plaster of Paris combination with /without platelet-rich plasma in the treatment of bone defects around implants.Int J Oral Maxillofac Implants. 2012; 17(1):86-94.

[33] Anitua E,Sanchez M,Nurden AT,et al.New insights into and novel applications for platelet-rich fibrin therapies.Trends Biotechnol.2006;24(5):227-234.

[34] Jensen TB,Rahbek O,Overgaard S,et al.Platelet-rich plasma and fresh frozen bone allograft as enhancement of implant fixation: An experimental study in dogs. J Orthop Res. 2004;22(3):653-658.

[35] Jensen TB,Rahbek O,Overgaard S,et al.Platelet-rich plasma and fresh frozen bone allograft as enhancement of implant fixation: An experimental study in dogs.J Orthop Res.2004;22(3):653-658.

[36] Kitoh H,Kitakoji T,Tsuchiya H,et al.Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis--a preliminary result of three cases. Bone. 2004;35(4):892-898.

[37] Robiony M,Polini F,Costa F,et al.Osteogenesis distraction and platelet-rich plasma for bonerestoration of the severely atrophic mandible: preliminary results.J Oral Maxillofac Surg.2002;60(6):630-635.

[38] Zechner W,Tangl S,Tepper G,et al.Influence of platelet-rich plasma on osseous healing of dental implants: a histologic and hisgomorphometric study in minipigs.Int J Oral Maxillofac Implants. 2003;18(1): 15-22.

[39] Choi BH,Zhu SJ,Kim BY,et al.Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study.Int J Oral Maxillofac Surg.2005;34(4):420-424.

[40] Kim SG,Chung CH,Kim YK,et al.Use of particulate dentin-plaster of Paris combination with /without platelet-rich plasma in the treatment of bone defects around implants.Int J Oral Maxillofac Implants. 2002; 17(1):86-94.

Anitua E, Sanchez M,Nurden AT,et al.New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol.2006;24(5):227-23