Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (14): 2040-2045.doi: 10.3969/j.issn.2095-4344.2016.14.009

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Morphology and proliferation of bone marrow mesenchymal stem cells and expressions of CD34 and CD44 under stomach cancer microenvironment

Wu Gao-feng1, Liu Xi-ping1, Yang Bai-lin1, Li Pei-qing1, Ming Hai-xia1, Zhang Wei2   

  1. 1Basic Medicine School of Gansu University of Chinese Medicine, Lanzhou 73000, Gansu Province, China; 2the First Affiliated Hospital of Lanzhou University, Lanzhou 73000, Gansu Province, China
     
  • Received:2016-02-21 Online:2016-04-01 Published:2016-04-01
  • Contact: Liu Xi-ping, M.D., Professor, Basic Medicine School of Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, China
  • About author:Wu Gao-feng, Studying for master’s degree, Basic Medicine School of Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81260525

Abstract:

BACKGROUND: Gastric cancer mesenchyal stem cells from clinical stomach cancer specimens and tumorigenic tissues in nude mice are similar to the bone marrow mesenchymal stem cells in biological characteristics, which have been proved to be an important component of tumor microenvironment to promote tumor growth. It is speculated that biological characteristic of bone marrow mesenchymal stem cells may change in stomach cancer microenvironment.

OBJECTIVE: To observe the effect of stomach cancer microenvironment on morphology and proliferation of bone marrow mesenchymal stem cells and expressions of CD34 and CD44.
METHODS: Rat bone marrow mesenchymal stem cells were cultured alone as control group. In the test group, rat bone marrow mesenchymal stem cells were co-cultured with human stomach cancer BGC-823 cells using Transwell chamber assay to establish the stomach cancer microenvironment. Then, cell morphology, proliferation, cell cycle and CD34, CD44 expressions were observed and detected using inverted phase contrast microscope, MTT assay, and flow cytometry, respectively.
RESULTS AND CONCLUSION: In the test group, bone marrow mesenchymal stem cells were similar to human stomach cancer cells BGC-823 that arranged disorderly and irregularly, were interconnected loosely, became thinner and longer, and grew in clusters with smaller nuclei. The cell proportion in G1 phase significantly decreased, but that in S and G2/M phases significantly increased (P < 0.01, P < 0.05). The positive rate of CD44 significantly declined, and the CD34 expression significantly raised (P < 0.01). In conclusion, stomach cancer microenvironment by non-contact co-culture with BCG-823 cells has an obvious effect on the morphology, proliferation and surface antigens expressions of bone marrow mesenchymal stem cells that will tend to be malignant gastric cancer cells.
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程 

Key words: Mesenchymal Stem Cells, Cell Proliferation, CD34, CD44, Tissue Engineering