Effects of allogeneic versus autologous serum on the proliferation of bone marrow stromal stem cells

doi:10.3969/j.issn.2095-4344.2015.45.005

Abstract

Abstract:

BACKGROUND: Fetal bovine serum as nutritional support is often used in the traditional cell culture. Consequently, a host of potential problems such as the spread of disease and immunological reactions exist. To find a suitable fetal bovine serum substitute and to establish a culture system of human bone marrow stromal stem cells in vitro which has been standardized, safe and efficient has just started.

OBJECTIVE: To investigate the effects of different serums on proliferation of bone marrow stromal stem cells in vitro.

METHODS: Bone marrow stromal stem cells were obtained from adult bone marrow, which were cultured in DMEM containing 10% AB serum, 10% autologous serum, or 10% fetal bovine serum. Cells at passage 3 were used in this study.

RESULTS AND CONCLUSION: The cell confluence in the AB serum group was earlier than that in the fetal bovine serum group and autologous serum group. Human bone marrow stromal stem cells maintained the phenotypes of bone marrow stem cells in three serums detected by flow cytometry. AB serum group showed the highest fluorescence intensity and the most efficiency of cell proliferation which examined by the AlamarBlue assay. Apoptosis rate was < 5% in all the three groups, and cells grew well in these serums. Alkaline phosphatase, calcium nodules and oil red O staining showed that the cells maintained the osteogenesis and adipogenesis capacity in the three groups. AB serum was found to have a better effect on proliferation capability of cells than fetal bovine serum and autologous serum. Taken together, AB serum is expected to be a substitute of fetal bovine serum to build an in vitro culture system of adult bone marrow stromal stem cells that accord with the clinical requirements of bone tissue engineering.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Serum, Cells, Cultured, Cell Proliferation, Tissue Engineering

Li Fang-guo, Lu Yan-dong, Cui Meng, Zhao Jia-guo, Yang Qiang, Wang Lei, Pei Guo-xian, Sun Jie. Effects of allogeneic versus autologous serum on the proliferation of bone marrow stromal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7242-7248.

Figures/Tables 4

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