Advances in serum-free culture systems of human embryonic stem cells

doi:10.3969/j.issn.2095-4344.2015.41.027

Abstract

Abstract:

BACKGROUND: Human embryonic stem cells are able to self-renew indefinitely and have the capacity to differentiate into all three germ layers (ectoderm, endoderm and mesoderm). These properties imply great potential in the basic research and clinical application, including regenerative medicine, drug screening and toxins, early human embryo, cell transplantation, gene therapy, etc. However, it is a substantial challenge to develop efficient techniques for their large-scale culture under defined conditions, and for controlling and directing their differentiation. For therapeutic purposes, many scholars are trying to establish methods for maintaining pluripotency in defined xeno-free conditions and scalable culture systems.
OBJECTIVE: To discuss the progress of serum-free culture systems in human embryonic stem cell research
reported in recent years and to highlight the challenges and advances being made towards the development of serum-free and xeno-free culture systems suitable for therapeutic applications.
METHODS: A computer-based search of CNKI and PubMed academic database was performed for articles addressing serum-free culture systems of human embryonic stem cells published from 2008 to 2015. Repetitive and old articles were excluded. Finally, 58 articles were summarized.
RESULTS AND CONCLUSION: Several groups have attempted to exclude individual animal components by using feeder-free matrices, feeder cells of human origin, or defined xeno-free media, aiming to select a suitable matrix and medium that can minimize or not use heterologous components, in order to obtain cell lines at clinical level. However, the current cell products are far from clinical application. There are still many problems to be solved, such as standardization, normalization and individualization of cell products. With the normative development of stem cell research and industry, human embryonic stem cell products are expected to be widely used in clinic.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Embryonic Stem Cells, Culture Media, Serum-Free, Culture Media, Conditioned, Tissue Engineering

Zhang Zhe, Zeng Xian-zhuo, Lu Fei, Guo Ming-hui, Dong Hui-jun . Advances in serum-free culture systems of human embryonic stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(41): 6711-6717.

Figures/Tables 2

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