Platelet-derived endothelial cell growth factor transfection of adipose-derived mesenchymal stem cells promotes vascularization of fat grafts

doi:10.3969/j.issn.2095-4344.2015.41.008

Abstract

Abstract:

BACKGROUND: Platelet-derived endothelial cell growth factor (PD-ECGF) can promote revascularization in fat transplantation.
OBJECTIVE: To explore the dual effects of PD-ECGF and adipose-derived mesenchymal stem cells on the survival rate of fat grafts.
METHODS: (1) Adipose-derived mesenchymal stem cells were isolated from the inguinal subcutaneous fat of New Zealand white rabbits, and then cultured. Passage 3 adipose-derived mesenchymal stem cells were divided into experimental group (Lenti-PD-ECGF-EGFP transfected adipose-derived mesenchymal stem cells), control group (Lenti-EGFP transfected adipose-derived mesenchymal stem cells) and blank group (adipose-derived mesenchymal stem cells with no transfection). (2) Lenti-PD-ECGF-EGFP transfected adipose-derived mesenchymal stem cells were cultured in DMEM complete medium, and then mixed with fat tissues as group A; adipose-derived mesenchymal stem cells with no transfection were cultured in DMEM complete medium and then mixed with fat tissues as group B; DMEM complete medium with no cells served as group C. Then, the grafts in groups A, B, C were respectively injected subcutaneously into the upper left, lower left and upper right parts of the rabbits’ black.
RESULTS AND CONCLUSION: (1) In the experimental group, PD-ECGF mRNA and protein expressions were significantly higher than those in the control and blank groups (P < 0.05), and cell proliferation was also the fastest.
(2) Graft weight and the number of capillaries were greater in group A than groups B and C. These findings indicate that PD-ECGF transfection of adipose-derived mesenchymal stem cells not only can continuously express the PD-ECGF protein, but also can promote the proliferation of adipose-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Transfection, Lentivirus Infections, Capillaries, Mesenchymal Stem Cell Transplantation, Tissue Engineering

CLC Number:

R394.2

San Guang, Song Jia. Platelet-derived endothelial cell growth factor transfection of adipose-derived mesenchymal stem cells promotes vascularization of fat grafts[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.41.008.

Figures/Tables 8

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