Variations of brain edema and neurological function of rat models of cerebral infarction after hyperbaric oxygen therapy

doi:10.3969/j.issn.2095-4344.2015.40.006

Abstract

Abstract:

BACKGROUND: Several studies have suggested that hyperbaric oxygen could better protect cranial nerve and brain cells. Hyperbaric oxygen therapy can make oxygen partial pressure rapidly diffusing toward relatively hypoxic brain tissue, so as to increase blood oxygen content in the brain tissue, reduce brain edema and promote the recovery of brain function.

OBJECTIVE: To observe the effects of hyperbaric oxygen therapy on brain tissue swelling in rats after middle cerebral artery occlusion, and discuss the possible mechanism of action underlying the neuroprotective effects of hyperbaric oxygen therapy in rats with cerebral infraction.

METHODS: Sixty adult female rat models of cerebral infarction were successfully established by middle cerebral artery occlusion using suture method and then randomly divided into the sham, cerebral infarction and hyperbaric oxygen therapy groups (n=20 rats/group). At 3 days after middle cerebral artery occlusion, apoptosis of nerve cells in the infract area of rats in each group was detected by TUNEL method. At 72 hours after middle cerebral artery occlusion, the gene transcription and protein expression of aquaporin 4/9 and matrix metalloproteinases 9/2 in the peri-infarct area were detected by RT-PCR and western blot analysis. The pathomorphological change in the infract area was observed by hematoxylin-eosin staining. The expression level of glial fibrillary acidic protein was detected by immunohistochemistry. At 24 hours and 3 days after hyperbaric oxygen therapy and at 1 and 2 weeks after middle cerebral artery occlusion, neurological behaviors were evaluated using Longa behavioral scores.

RESULTS AND CONCLUSION: After 1, 2 days of hyperbaric oxygen therapy, Longa behavioral scores in the hyperbaric oxygen therapy group were significantly lower than those in the cerebral infarction group (P < 0.05). At 3 days after middle cerebral artery occlusion, cell apoptosis index in the hyperbaric oxygen therapy group was significantly lower than that in the cerebral infarction group (P < 0.05). At 72 hours after middle cerebral artery occlusion, the aquaporin 4/9, matrix metalloproteinases 9/2 gene and protein expression in the hyperbaric oxygen group were significantly lower than those in the cerebral infarction group (P < 0.05). These results suggest that hyperbaric oxygen therapy can play its protective role by decreasing apoptosis of nerve cells in the infarct area and lessening the edema of brain tissue in rats with cerebral infarction.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Cerebral Infarction, Hyperbaric Oxygen, Nerve Regeneration

CLC Number:

R318

Tian Xuan. Variations of brain edema and neurological function of rat models of cerebral infarction after hyperbaric oxygen therapy[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6423-6428.

Figures/Tables 2

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