Vascular endothelial growth factor 165 transfection promotes proliferation of adipose-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.36.008

Abstract

Abstract:

BACKGROUND: Vascular endothelial growth factor is the most important pro-angiogenic factor, which can promote revascularization and survival of fat grafts during fat transplantation.
OBJECTIVE: To investigate the effect of vascular endothelial growth factor 165 transfection on the proliferation of adipose-derived mesenchymal stem cells.
METHODS: Recombinant vascular endothelial growth factor 165 mRNA fragment was transmitted into adenovirus pAdEasy-1 systems that were packaged to measure viral titer (experimental group). Empty adenovirus was also packaged as control group. Two kinds of packaged adenovirus solution were transferred into adipose-derived mesenchymal stem cells at the best multiplicity of infection=100. Cells with no transfection served as blank group. RT-PCR and western blot methods were used to detect the expression of vascular endothelial growth factor 165 at mRNA and protein levels; MTT method was adopted to detect cell proliferation.
RESULTS AND CONCLUSION: The expressions of vascular endothelial growth factor 165 mRNA and protein were significantly higher in the experimental group than the control and blank groups (P < 0.05). The division and proliferation of transfected adipose-derived mesenchymal stem cells were increased significantly in the experimental group, which was significantly different from the control and blank groups (P < 0.05). These finding indicate that vascular endothelial growth factor 165 transfection cannot only sustain the target protein expression of adipose- derived mesenchymal stem cells, but also promote the proliferation of adipose-derived mesenchymal stem cells remarkably.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Adipose Tissue, Mesenchymal Stem Cells, Vascular Endothelial Growth Factors, Cell Proliferation

CLC Number:

R394.2

San Guang, Song Jia. Vascular endothelial growth factor 165 transfection promotes proliferation of adipose-derived mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5782-5788.

Figures/Tables 4

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