Expression of aromatase and estrogen-related receptors in human bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.36.004

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (36): 5758-5763.doi: 10.3969/j.issn.2095-4344.2015.36.004

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Expression of aromatase and estrogen-related receptors in human bone marrow mesenchymal stem cells

Wei Qiu-shi^{1, 2}, Chen Zhen-qiu^{1, 3}, He Wei^{1, 3}, Deng Wei-min², Wang Hai-bin^{1, 3}, Huang Shi-jin³, Guo Cheng³

1Department of Orthopedics, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510407, Guangdong Province, China; 2Postdoctoral Station of Rehabilitation Department, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China; 3Key Laboratory of Osteology and Traumatology of TCM, National Key Disciplines, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China

Online:2015-09-03 Published:2015-09-03
Contact: He Wei, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510407, Guangdong Province, China; Key Laboratory of Osteology and Traumatology of TCM, National Key Disciplines, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China
About author:Wei Qiu-shi, M.D., Department of Orthopedics, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510407, Guangdong Province, China; Postdoctoral Station of Rehabilitation Department, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81302994 and 81473697; the Natural Science Foundation of Guangdong Province, No. S2013040014927

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Abstract

Abstract:

BACKGROUND: Estrogen signaling pathway for interaction between aromatase and estrogen-related receptor may exist in bone marrow mesenchymal stem cells, which is used for regulating biological activity of bone marrow mesenchymal stem cells.

OBJECTIVE: To observe the expression of aromatase and estrogen-related receptors in adult bone marrow mesenchymal stem cells during osteogenic differentiation.

METHODS: Bone marrow mesenchymal stem cells were respectively cultured in low-glucose DMEM medium (control group) and osteogenic induction medium (induction group). Cell proliferation and calcium deposition were determined by MTT assay and alizarin red staining, respectively. The expression of aromatase, estrogen receptor α, estrogen receptor β, and estrogen-related receptor α during osteogenic differentiation were determined by real-time PCR and western blot analysis. Estradiol levels in supernatants and lysates were detected by ELISA method.

RESULTS AND CONCLUSION: In the induction group, the proliferation ability of bone marrow mesenchymal stem cells was the strongest at 72 hours of culture; while there were a great amount of calcium nodules formed at 21 days of culture. Results from PCR and western blot assay showed that the expression of aromatase and estrogen receptor α was improved in the induction group, but the expression of estrogen-related receptor α was inhibited. There was no difference in the expression of estrogen receptor β between the two groups. ELISA results indicated that the level of estradiol in the supernatant of induction group was the highest. These findings indicate that aromatase, estrogen receptor α, estrogen receptor β and estrogen-related receptor α are all involved in osteogenesis of bone marrow mesenchymal stem cells. Moreover, estradiol can be synthesized and secreted in bone marrow mesenchymal stem cells, and most likely, promote the osteogenic differentiation of bone marrow mesenchymal stem cells by related receptor pathway.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Receptors, Estrogen, Cell Differentiation

CLC Number:

R394.2

Wei Qiu-shi, Chen Zhen-qiu, He Wei, Deng Wei-min, Wang Hai-bin, Huang Shi-jin, Guo Cheng. Expression of aromatase and estrogen-related receptors in human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5758-5763.

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Expression of aromatase and estrogen-related receptors in human bone marrow mesenchymal stem cells

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