Effect of bone xenograft extract on biological characteristics of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.36.003

Abstract

Abstract:

BACKGROUND: How to preserve the biological properties and osteogenic activity of bone xenograft materials after antigen removal has become one of the hot spots.

OBJECTIVE: To investigate the effect of bone xenograft extract on proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cells.

METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawely rats were cultured respectively in bone xenograft extract (experimental group) and DMEM/F12 medium containing 10% fetal bovine serum (control group). MTT, Transwell chamber and flow cytometry were used to detect cell proliferative ability, migration ability and cell cycle changes. Passage 3 bone marrow mesenchymal stem cells were selected in the two groups and cultured in osteogenic induction medium. At 7 and 14 days of osteogenic induction, alkaline phosphatase staining was done; at 21 days of osteogenic induction, alizarin red staining was performed to observe calcium nodule formation.

RESULTS AND CONCLUSION: After 1, 3, 5, 7 days of culture, the cell proliferative activity and cell cycle had no significant difference between the two groups, but the number of migrating cells was significantly lower in the control group than the experimental group (P < 0.05). At 7 and 14 days of culture, the alkaline phosphatase level was significantly higher in the experimental group than the control group (P < 0.05); at 21 days of culture, calcium nodes formed in both two groups, but the calcium nodule area was larger in the experimental group than the control group. These findings indicate that bone xenograft materials have no cytotoxicity but possess good cytocompatibility, which can promote the proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Biocompatible Materials, Cell Differentiation

CLC Number:

R394.2

Li Fei, Chen Yang, Wang Zhan-chang. Effect of bone xenograft extract on biological characteristics of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5753-5757.

Figures/Tables 2

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