Chuanglingye-loaded collagen promotes chronic wound healing

doi:10.3969/j.issn.2095-4344.2015.34.021

Abstract

Abstract:

BACKGROUND: It has been shown that Chuanglingye has good anti-inflammatory effects, inhibits platelet aggregation and thrombus and improve wound healing.

OBJECTIVE: To investigate the effects of Chuanglingye-loaded collagen on regulating inflammation and healing process of chronic wounds.

METHODS: Forty rats were selected to make chronic wound models and randomly divided into four groups at 3 days after modeling: control group, collagen group, Chuanglingye group, Chuanglingye-loading collagen group, 10 rats in each group, respectively treated with 0.2 mL normal saline, 0.2 mL Chuanglingye, collagen and Chuanglingye-loaded collagen. The size of wounds was measured when modeling and at 3, 7 and 15 days after modeling. Amount of white blood cells, interleukin-6 level and tumor necrosis factor-α level in wound exudates were detected. The levels of hydroxyproline and matrix mentalloproteases (MMPs), including MMP-1, MMP-2, MMP-9, in the granulation tissue, were also detected.

RESULTS AND CONCLUSION: At 3, 7, 15 days after modeling, the amount of white blood cells and levels of interleukin-6, tumor necrosis factor-α, MMP-1, MMP-2, MMP-9 were significantly lower in the Chuanglingye-loaded collagen group and Chuanglingye group compared to the control group and collagen group (P < 0.01, P < 0.05). But there was no significant difference between Chuanglingye-loaded collagen group and Chuanglingye group. The level of hydroxyproline in the granulation tissue was significantly higher in the Chuanglingye-loaded collagen group than the other three groups at 3, 7, 15 days after modeling (P < 0.05), and the size of wound in the Chuanglingye-loaded collagen group was significantly lower than that in the other three groups at 15 days after modeling (P < 0.01). In addition, no significant difference was found among the four groups at 15 days after modeling. These findings indicate that Chuanglingye-loaded collagen can significantly depress proliferation and infiltration of inflammatory cells on the wound surface, decrease levels of inflammatory factors and MMPs in wound exudates, and thus promote the healing of chronic wounds.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Collagen, Inflammation, Interleukin-6, Tumor Necrosis Factor-alpha, Matrix Metalloproteinases

CLC Number:

R318

Yao Chang, Jiang Hong, Xu Yan-lei, Zhu Yong-kang. Chuanglingye-loaded collagen promotes chronic wound healing[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(34): 5518-5522.

Figures/Tables 6

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