Biocompatibility of poly-L-lactic acid, a new synthetic material for skin burn

doi:10.3969/j.issn.2095-4344.2015.34.014

Abstract

Abstract:

BACKGROUND: Recent studies have shown that poly-L-lactic acid (PLLA) polymer has good biocompatibility and can be directly involved in human metabolism without any side effects, which is a polymer material that can be used as bioscaffolds.

OBJECTIVE: To explore the biocompatibility of PLLA.

METHODS: The moisture absorption rate of collagen composite and PLLA was detected. Cell morphology observation was carried to detect the growth state of C3H10T1/2 cells that grew in normal HDMEM medium, HDMEM medium+dimethyl sulfoxide, HDMEM medium+collagen composite extract, HDMEM medium+PLLA extract for 72 hours. MTT kit was used to detect the relative proliferation rate of the C3H10T1/2 cell lines that grew in the above-mentioned media and the toxicity evaluations were based on the material toxicity criteria. Hemolysis degree was measured in rabbit blood samples containing normal saline, distilled water, PLLA extract and collagen composite extract. The allergic reaction and pyrogen reaction in rabbits were observed through injection of normal saline, distilled water, PLLA extract and collagen composite extract via the ear vein. Collagen composite and PLLA were respectively implanted subcutaneously into the rabbits, and after 4 weeks, the levels of interleukin-10 and interleukin 23 in serum were detected.

RESULTS AND CONCLUSION: The moisture absorption rates per unit area and per unit mass of collagen composite material were lower than those of PLLA synthetic materials (P < 0.05). C3H10T1/2 cells grew well in the PLLA extract, with a higher relative growth rate and lower cytotoxicity (grade I). Compared with the collagen composite materials, the PLLA synthetic materials had a lower hemolysis ratio and less inflammatory reactions (P < 0.05); and there were no allergic reaction and pyrogen reaction. These findings indicate that the PLLA scaffold materials for burned skin have good absorbing fluid performance and biocompatibility.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Collagen, Materials Testing, Biocompatible Materials

CLC Number:

R318

Deng Li-huan. Biocompatibility of poly-L-lactic acid, a new synthetic material for skin burn[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(34): 5480-5484.

Figures/Tables 6

References

[1] Li J,Hong J,Zheng Q, et al.Repair of rat cranial bone defects with nHAC/PLLA and BMP-2-related peptide or rhBMP-2.J Orthop Res.2011;29(11):1745-1752.
[2] Yuan Y,Jin X,Fan Z,et al.In vivo degradation of copolymers prepared from L-lactide, 1,3-trimethylene carbonate and glycolide as coronary stent materials. J Mater Sci Mater Med. 2015;26(3):139.
[3] Pauck RG,Reddy BD.Computational analysis of the radial mechanical performance of PLLA coronary artery stents.Med Eng Phys.2015;37(1):7-12.
[4] Busch R,Strohbach A,Rethfeldt S,et al.New stent surface materials: the impact of polymer-dependent interactions of human endothelial cells, smooth muscle cells, and platelets.Acta Biomater.2014;10(2):688-700.
[5] Inoue M,Sasaki M,Katada Y,et al.Poly-(L-lactic acid) and citric acid-crosslinked gelatin composite matrices as a drug-eluting stent coating material with endothelialization, antithrombogenic, and drug release properties.J Biomed Mater Res A.2013;101(7): 2049-2057.
[6] Flege C,Vogt F,Höges S,et al.Development and characterization of a coronary polylactic acid stent prototype generated by selective laser melting.J Mater Sci Mater Med.2013;24(1): 241-255.
[7] Kim HI,Ishihara K,Lee S,et al. Tissue response to poly (L-lactic acid)-based blend with phospholipid polymer for biodegradable cardiovascular stents.Biomaterials. 2011;32(9):2241-2247.
[8] Bruining N,de Winter S,Roelandt JR,et al.Monitoring in vivo absorption of a drug-eluting bioabsorbable stent with intravascular ultrasound-derived parameters a feasibility study.JACC Cardiovasc Interv.2010;3(4):449-456.
[9] Lewis RA.Ab interno approach to the subconjunctival space using a collagen glaucoma stent. J Cataract Refract Surg. 2014;40(8):1301-1306.
[10] Zhao N,Workman B,Zhu D.Endothelialization of novel magnesium-rare earth alloys with fluoride and collagen coating. Int J Mol Sci.2014;15(4):5263-5276.
[11] Li J,Zhang K,Chen H,et al.A novel coating of type IV collagen and hyaluronic acid on stent material-titanium for promoting smooth muscle cell contractile phenotype. Mater Sci Eng C Mater Biol Appl.2014;38:235-243.
[12] Soltysiak P,Höllwarth ME,Saxena AK.Comparison of suturing techniques in the formation of collagen scaffold tubes for composite tubular organ tissue engineering. Biomed Mater Eng.2010;20(1):1-11.
[13] Martínez de Arenaza I,Obarzanek-Fojt M,Sarasua JR,et al. Pyrene-end-functionalized poly(L-lactide) as an efficient carbon nanotube dispersing agent in poly(L-lactide): mechanical performance and biocompatibility study. Biomed Mater. 2015; 10(4):045003.
[14] Cai Q,Shi Y,Shan D,et al.Osteogenic differentiation of MC3T3-E1 cells on poly (l-lactide)/Fe3O4 nanofibers with static magnetic field exposure. Mater Sci Eng C Mater Biol Appl.2015; 55:166-173.
[15] Yang DZ,Chen AZ,Wang SB,et al.Preparation of poly(L-lactic acid) nanofiber scaffolds with a rough surface by phase inversion using supercritical carbon dioxide .Biomed Mater.2015; 10(3):035015.
[16] Hu Y,Darcos V,Monge S,et al. Thermo-responsive drug release from self-assembled micelles of brush-like PLA/PEG analogues block copolymers.Int J Pharm.2015;491(1-2): 152-161.
[17] Yang Y,Wu J,Jin G,et al.Research of osteoblastic induced rat bone marrow mesenchymal stem cells cultured on β-TCP/PLLA porous scaffold. Int J Clin Exp Med. 2015;8(3):3202-3209.
[18] Di Bonito P,Petrone L,Casini G,et al.Amino-functionalized poly(l-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development.Int J Nanomedicine.2015;10:3447-3458.
[19] Yu Y,Lü X,Ding F. microRNA regulatory mechanism by which PLLA aligned nanofibers influence PC12 cell differentiation.J Neural Eng.2015;12(4):046010.
[20] Kowalski W,Dammer M,Bakczewitz F,et al.In-situ investigation of stress conditions during expansion of bare metal stents and PLLA-coated stents using the XRD sin(2)ψ-technique.J Mech Behav Biomed Mater.2015; 49:23-29.
[21] Lin JH,Chen CK,Wen SP,et al.Poly-L-lactide/sodium alginate/chitosan microsphere hybrid scaffolds made with braiding manufacture and adhesion technique: Solution to the incongruence between porosity and compressive strength. Mater Sci Eng C Mater Biol Appl.2015;52:111-120.
[22] Pan P,Han L,Bao J,et al.Competitive Stereocomplexation, Homocrystallization, and Polymorphic Crystalline Transition in Poly(l-lactic acid)/Poly(d-lactic acid) Racemic Blends: Molecular Weight Effects.J Phys Chem B.2015;119(21):6462-6470.
[23] Mashhadikhan M,Soleimani M,Parivar K,et al. ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties. Avicenna J Med Biotechnol. 2015;7(1):32-38.
[24] Liu M,Huang G,Cong Y,et al.The preparation and characterization of micelles from poly(γ-glutamic acid)-graft- poly(L-lactide) and the cellular uptake thereof. J Mater Sci Mater Med.2015;26(5):187.
[25] Wach RA,Adamus A,Kowalska-Ludwicka K,et al.In vivo evaluation of nerve guidance channels of PTMC/PLLA porous biomaterial.Arch Med Sci.2015;11(1):210-219.
[26] Zhang Z,Lv Q,Gao X,et al.pH-Responsive Poly(ethylene glycol)/Poly(L-lactide) Supramolecular Micelles Based on Host-Guest Interaction.ACS Appl Mater Interfaces. 2015 ; 7(16): 8404-8411.
[27] Lizundia E,Vilas JL,León LM.Crystallization, structural relaxation and thermal degradation in Poly (L-lactide)/cellulose nanocrystal renewable nanocomposites. Carbohydr Polym.2015;123: 256-265.
[28] Frydrych M,Román S,MacNeil S,et al.Biomimetic poly(glycerol sebacate)/poly(l-lactic acid) blend scaffolds for adipose tissue engineering. Acta Biomater.2015;18:40-49.
[29] Uchida S,Utsunomiya H,Taketa T,et al.Arthroscopic fragment fixation using hydroxyapatite/poly-L-lactate Acid thread pins for treating elbow osteochondritis dissecans.Am J Sports Med. 2015;43(5):1057-1065.
[30] Stein P,Vitavska O,Kind P,et al.The biological basis for poly-L-lactic acid-induced augmentation.J Dermatol Sci.2015; 78(1):26-33.