Femtosecond laser promotes the proliferation and differentiation of human gingival fibroblasts on pure titanium surface

doi:10.3969/j.issn.2095-4344.2015.34.004

Abstract

Abstract:

BACKGROUND: Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation.

OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface.

METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO₂ particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed.

RESULTS AND CONCLUSION: There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cells were more in the femtosecond laser group and TiO₂ particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group followed by TiO₂ particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Titanium, Lasers, Surface Properties, Fibroblasts

CLC Number:

R318

Li Ju, Dong Xiu-hua. Femtosecond laser promotes the proliferation and differentiation of human gingival fibroblasts on pure titanium surface [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(34): 5428-5432.

Figures/Tables 3

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