Paracrine effect of chondrocytes on gene expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in osteoblasts

doi:10.3969/j.issn.2095-4344.2015.33.012

Abstract

Abstract:

BACKGROUND: Cell co-culture can maximize the simulation of in vivo microenvironment. Cell scratch test and interleukin-1β can destroy the balance between matrix metalloproteinases (MMPs) and matrix metalloproteinase inhibitors (TIMPs), resulting in extracellular matrix degradation of the articular cartilage, functional disorders of chondrocytes and articular cartilage degeneration.

OBJECTIVE: To study the effect of interleukin-1β on migration, MMP and TIMP expression of chondrocytes co-cultured with osteoblast supernatant in vitro.

METHODS: There were three groups: chondrocyte monoculture group, osteoblast+chondrocyte group (co-culture group), osteoblast+chondrocyte+interleukin-1β group (interleukin-1β group). Cell scratch test was conducted to observe the migration of chondrocytes within 24 hours. Semi-quantitative PCR test was used to detect the changes in expressions of MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1, TIMP-2, TIMP-3, TIMP-9 in chondrocytes within 24 hours.

RESULTS AND CONCLUSION: Compared with the monoculture group, cell migration rate of the other two groups were increased significantly (P < 0.01). Compared with the monoculture group, the gene expressions of MMP-1, MMP-2, MMP-3 and MMP-9 were increased significantly in the coculture group (P < 0. 05); the gene expressions of MMP-1, MMP-3, MMP-9 were increased significantly in the interleukin-1β group (P < 0. 01). Compared with monoculture group, the gene expression of TIMP-1 was increased significantly (P < 0. 01), but the gene expressions of TIMP-3 and TIMP-4 were declined significantly (P < 0. 05) in the other two groups. These findings indicate that co-culture of chondrocytes with osteoblasts can promote chondrocytes migration, enhance gene expression of chondrocytes MMP-1, MMP-2, MMP-3, MMP-9 and regulate gene expression of TIMPs family. Interleukin-1β inhibits the migration of chondrocytes co-cultured with osteoblasts and gene expression of TIMPs family.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Chondrocytes, Osteoblast, Matrix Metalloproteinases

CLC Number:

R318

Guan Peng, Zhao Wei, Zhang Quan-you, Xie Jing, Yin Li-jun, Zhao Hu-cheng, Xu Jian-wen. Paracrine effect of chondrocytes on gene expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(33): 5306-2311.

Figures/Tables 4

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