Rapid production of tissue-engineered bone based on two kinds of adult stem cells in bone fusion

doi:10.3969/j.issn.2095-4344.2015.32.025

Abstract

Abstract:

BACKGROUND: Despite tissue repair with a kind of stem cells has achieved great outcomes, but the combination of two or more kinds of stem cells to construct tissue-engineered bone is still rare.

OBJECTIVE: To evaluate the efficacy of tissue-engineered bone constructed based on combining adipose-derived stem cells with bone marrow mesenchymal stem cells and allogenic bone in rabbit posterior intertransverse lumbar interbody fusion models.

METHODS: Seventy-five New Zealand rabbits were randomly divided into five groups. L5,6 interbody fusion models were made in each group. Then, these five groups were respectively treated as follows (1) group A: bone marrow mesenchymal stem cells/allograft iliac bone with adipose-derived stem cells; (2) group B: bone marrow mesenchymal stem cells/allograft iliac bone with normal saline; (3) group C: allograft iliac bone with adipose-derived stem cells; (4) group D: allograft iliac bone with normal saline; group E: autologous iliac bone alone. Each group was detected by PET/CT to contrast the standard uptake value at 1, 3, 5 weeks postoperatively.

RESULTS AND CONCLUSION: PET/CT images showed varying degrees of bone fusion and bone metabolism enhancement in the five groups. With time, the standard uptake value was increased in each group, and except the group D, the standard uptake value was higher in all the groups at 3, 5 weeks than at 1 week (P < 0.05). There was no difference in the standard uptake value of all the five groups at weeks 3 and 5 after operation (P > 0.05). The standard uptake value of the group A was better than that in the group B and group E (P < 0.05). Taken together, the combination of adipose-derived stem cells and bone marrow mesenchymal stem cells for rapid production of tissue-engineered bone has good osteogenesis and vascularization.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Adipose Tissue, Mesenchymal Stem Cells, Tissue Engineering, Transplantation, Homologous

CLC Number:

R394.2

Yin Jian, Yang Yi, Yang Xiao-feng, Xiao Wei, Chai Li-ming, Yuan Hong, Jin Ge-le. Rapid production of tissue-engineered bone based on two kinds of adult stem cells in bone fusion [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5220-5224.

Figures/Tables 4

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