Regulating osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells by extracellular adenosine triphosphate

doi:10.3969/j.issn.2095-4344.2015.32.001

Abstract

Abstract:

BACKGROUND: Impaired balance between osteogenesis and adipogenesis of bone marrow mesenchymal stem cells is a crucial pathological mechanism of osteoporosis. Mechanical loads applied to bone tissue can increase bone formation and improve bone strength, and meanwhile lead to the release of extracellular nucleotides, such as adenosine triphosphate.
OBJECTIVE: To determine the effects of adenosine triphosphate on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells and to investigate the underlying mechanisms.
METHODS: The effect of adenosine triphosphate (10, 50, 250 μmol/L) on differentiation of bone marrow mesenchymal stem cells were measured by osteogenic and adipogenic related genes expression, alizarin red staining and oil red O staining. The activation of ERK1/2 signaling pathway by adenosine triphosphate was tested using western blot assay.
RESULTS AND CONCLUSION: Incubation of bone marrow mesenchymal stem cells with adenosine triphosphate resulted in the dose-dependent increase of osteogenic genes expression and calcium deposition, and inhibition of adipogenic genes expression and lipid droplet formation, but had no effects on cell proliferation. Adenosine triphosphate activated ERK1/2 signaling pathway, and U0126 as an ERK1/2 inhibitor restrained the effect of adenosine triphosphate on the differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Adenosine Triphosphate, Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation

CLC Number:

R394.2

Li Wen-kai, Zhang Ying-chi, Wei Sheng, Yang Yong, Wu Hua. Regulating osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells by extracellular adenosine triphosphate[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5085-5091.

Figures/Tables 3

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