Influence of NGF-PEG-PLGA-NPs on neuronal differentiation of neural stem cells and PI3K/Akt signaling pathway

doi:10.3969/j.issn.2095-4344.2015.28.027

Abstract

Abstract:

BACKGROUND: Our previous studies confirmed that NGF-PEG-PLGA-NPs has good sustained release effect and biological activity in vitro, and can induce the differentiation of PC12 cells into neuron-like cells.
OBJECTIVE: To investigate the feasibility of neuronal differentiation of neural stem cells from septal area of fetal brain induced by NGF-PEG-PLGA-NPs and its influence on PI3K/Akt signaling pathway.
METHODS: According to optimization prescription, NGF-PEG-PLGA-NPs were prepared by multiple emulsion solvent diffusion method. Neural stem cells were induced to neuronal differentiation in six groups, including control group, NGF group, NGF-PEG-PLGA-NPs group, LY294002 group, LY294002+NGF group and LY294002+NGF-PEG-PLGA-NPs group. Neurons were identified by immunofluorescence, while phosphorylation levels of Akt in PI3K/Akt signaling pathway were detected by western blotting.
RESULTS AND CONCLUSION: The proportions of β-Tubulin III-positive neurons in control group, NGF group, NGF-PEG-PLGA-NPs group, LY294002 group, LY294002+NGF group and LY294002+NGF-PEG-PLGA-NPs group were (22.80±2.58)%, (35.80±3.98)%, (35.40±5.77)%, (26.60±3.87)%, (21.20±2.59)% and (25.80±7.22)%, respectively. There were no statistical differences in neuronal differentiation between NGF group and NGF-PEG-PLGA-NPs group
(P > 0.05), but the ratios of neural differentiation in the two groups were both higher than that in the other four groups
(P < 0.05). Western blotting results revealed that there were no statistical differences in Akt phosphorylation levels between NGF group and NGF-PEG-PLGA-NPs group (P > 0.05), but the phosphorylation levels of Akt were both higher than other four groups (P < 0.05). There were also no significant differences between LY294002+NGF and LY294002+NGF-PEG-PLGA-NPs groups and control group (P > 0.05), but the phosphorylation levels of Akt were higher than LY294002 group (P < 0.05). Results suggest that NGF-PEG-PLGA-NPs promoted neural differentiation of neural stem cells. The role might be related to increasing phosphorylation levels of Akt in PI3K/Akt signaling pathway.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Neural Stem Cells, Nerve Growth Factor, Nanoparticles

CLC Number:

R394.2

Chen Yan, Bao Guo-qing, Liu Fei-fei, Zhang Jun-du, Pan Cui-huan, Long Da-hong. Influence of NGF-PEG-PLGA-NPs on neuronal differentiation of neural stem cells and PI3K/Akt signaling pathway [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4576-4581.

Figures/Tables 3

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