Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (28): 4445-4449.doi: 10.3969/j.issn.2095-4344.2015.28.004

Previous Articles     Next Articles

Radix Astragali injection at appropriate concentrations enhances surface adhesion of bone marrow stromal cell

Wang Cai-xia1, Ren Ming-ji2, Cui Ming-yu2   

  1. 1Department of Anesthesiology, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 
    2Department of Histology and Embryology, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Cui Ming-yu, Professor, Master’s supervisor, Department of Histology and Embryology, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China
  • About author:Wang Cai-xia, Master, Chief physician, Department of Anesthesiology, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China

PDF

277

Abstract:

BACKGROUND: Several studies have demonstrated that many kinds of Chinese herbs for benefiting vital energy and enriching the blood secrete some cytokines by influencing bone marrow stromal cells to promote the differentiation and proliferation of hemopoietic stem cells or exert effects by promoting the adhesion of bone marrow stromal cells and hemopoietic stem cells.

OBJECTIVE: To investigate the effects of Radix Astragali injection on the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 on the surface of mouse bone marrow stromal cells.
METHODS: Mouse bone marrow stromal cells were isolated by adherent culture of whole bone marrow. The morphology of mouse bone marrow stromal cells were observed using inverted phase microscopy, hematoxylin-eosin staining, optical microscopy and transmission electron microscopy. The optimal concentration at which Radix Astragali injection exhibited the strongest effects on promoting the proliferation of bone marrow stromal cells was detected using MTT assay. Expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 on the surface of mouse bone marrow stromal cells was detected using flow cytrometry after intervention by Radix Astragali injection.

RESULTS AND CONCLUSION: Inverted phase microscopy and optical microscopy showed that bone marrow stromal cells adhered to the wall of culture flask, exhibited a shuttle-shaped or irregular appearance with processes. Through transmission electron microscopy, organelles were abundant, such as rough endoplasmic reticulum, secretory vesicle, and mitochondria. Radix Astragali injection at 400 and 600 mg/L significantly promoted the proliferation of mouse bone marrow stromal cells (P < 0.05) and there was no significant difference in the cell proliferation between these two concentrations. Radix Astragali injection at 600 mg/L increased the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 on the surface of mouse bone marrow stromal cells. These findings suggest that Radix Astragali injection at appropriate concentrations can increase the surface adhesion of bone marrow stromal cells, thereby improving hemopoietic microenvironment. 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Astragalus membranaceus, Cell Adhesion Molecules, Intercellular Adhesion Molecule-1Bone Marrow, Mesenchymal Stem Cells, Astragalus membranaceus, Cell Adhesion Molecules, Intercellular Adhesion Molecule-1

CLC Number: