Liu Min, Master, Associate chief physician, Department of Urology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China

doi:10.3969/j.issn.2095-4344.2015.25.012

Abstract

Abstract:

BACKGROUND: Studies have shown that polyethylene polymer-synthesized ureteral stents have good biocompatibility in vivo, but the side effects are found to induce the body to produce inflammatory, pyrogenic, and allergic reactions. Therefore, studies have mainly aimed to explore the biocompatibility, toxic and side effects, inflammation of newly developed ureteral stents synthesized by L-lactic acid, glycolide, and barium sulfate.
OBJECTIVE: Based on the biocompatibility comparisons on cell toxicity test, inflammatory reaction, allergic reaction, pyrogen reaction and mortality after implantation between the usage of polyethylene material and L-lactic acid, glycolide, barium sulphate syntheses, to provide a better reference to introduce a new clinical ureteral stent material.
METHODS: There were three groups in the experiment: blank control group, new material group, polyethylene group. Cells were cultured in normal medium, leach liquid of L-lactic acid, glycolide, barium sulphate syntheses and leach liquid of polyethylene, respectively.
RESULTS AND CONCLUSION: The toxic effects were significantly enhanced in the urethral epithelial cells that grew in the leach liquids of two materials compared with the blank control group (P < 0.05), but the two kinds of materials had no significant statistical difference in the toxicity test (P > 0.05). Hematoxylin-eosin staining results showed that there were obvious inflammatory infiltrations in muscle tissue and the number of neutrophils and eosinophils were increased when the two kinds of materials were implanted in the rats for 2 and 6 weeks (P < 0.05), but the inflammatory infiltration and tissue damage in the new material group was less than that in the polyethylene group (P < 0.05). ELISA results showed that the serum concentrations of interleukin-10 and interleukin-23 were significantly increased when the two kinds of materials were implanted in the rats for 6 weeks (P < 0.05), but the content of inflammatory cytokines in the new material group was less than that in the polyethylene group (P < 0.05). In the new material group, there was only one rat that developed allergic reaction and there was no pyrogen reaction or death; however, in the polyethylene group, there were three rats with presence of allergic reaction and one rat with pyrogen reaction but no death. These findings indicate that the new ureteral stent material synthesized with L-lactic acid, glycolide and barium sulphate can reduce inflammation reactions, allergic reactions and pyrogen reactions to the body, and has better biocompatibility than polyethylene composite material.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Biocompatible Materials, Ureter, Stents

CLC Number:

R318

Liu Min, Yan Wei, Li Hui-feng, Zhu Tong-yu. Liu Min, Master, Associate chief physician, Department of Urology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(25): 3996-4001.

Figures/Tables 4

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