Preparation and performance of psoralen antibiotic biphasic ceramic bone

doi:10.3969/j.issn.2095-4344.2015.25.003

Abstract

Abstract:

BACKGROUND: In recent years, studies on the application of traditional Chinese medicine in bone tissue engineering are still at the initial stage in China. Reports about psoralen antibiotic biphasic ceramic bone have not been seen in bone tissue engineering research.
OBJECTIVE: To fabricate the psoralen antibiotic biphasic ceramic bone by vacuum evaporator and to observe the physicochemical properties, antibiotic activity and biocompatibility of the material.
METHODS: Biphasic ceramic bone was prepared by twice low-temperature calcining pig vertebrae combined with sodium pyrophosphate, and then the ceramic bone was immersed in chitosan/psoralen compound solution to prepare psoralen antibiotic biphasic ceramic bone. The surface morphology was observed by scanning electron microscope, and the compressive strength was tested. Antibiotic activity of the psoralen antibiotic biphasic ceramic bone on Escherichia coli and Staphylococcus aureus was tested by microbiological methods. Mouse osteoblasts MC3T3-E1 were co-cultured with the psoralen antibiotic biphasic ceramic bone for 4 and 8 days, and the cell adhesion and proliferation on the scaffold surface were observed under the scanning electron microscope.
RESULTS AND CONCLUSION: Psoralen antibiotic biphasic ceramic bone had natural pore structure with the trabecular bone, trabecular space and lumen system and exerted great antibiotic effect on Escherichia coli and Staphylococcus aureus. The compressive strength was (4.69±0.50) MPa. Mouse osteoblasts MC3T3-E1M could be adherent to the psoralen antibiotic biphasic ceramic bone and proliferate rapidly, suggesting the psoralen
antibiotic biphasic ceramic bone has good cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Psoralens, Ceramics, Escherichia coli

CLC Number:

R318

Xiu Xiao-guang, Zhang Shou-ping, Zhang Shu-hua, Wang Xiang-li. Preparation and performance of psoralen antibiotic biphasic ceramic bone[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(25): 3947-3951.

Figures/Tables 1

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