Biocompatibility of porcine bone marrow mesenchymal stem cells-derived bile duct endothelial cells with electrospun nanofibers

doi:10.3969/j.issn.2095-4344.2015.23.022

Abstract

Abstract:

BACKGROUND: Repair of extrahepatic biliary tract injury is a difficult problem in the abdominal surgery. Tissue-engineered extrahepatic biliary tract is an ideal selection for this problem. Construction of tissue-engineered extrahepatic biliary tract with excellent performance is a key to related studies.
OBJECTIVE: To investigate the biocompatibility of bile duct endothelial cells differentiated by porcine bone marrow mesenchymal stem cells with electrospun nanofibers.
METHODS: Porcine bone marrow mesenchymal stem cells were induced toward biliary tract endothelial cells, which were then identified by morphology and RT-PCR. Polylactic-co-glycolic acid (PLGA) nanofiber membranes were prepared by electrospinning. The morphology was determined by scanning electron microscopy and the short-term (2-week) in vitro degradation rate was determined. Adhesion and proliferation of biliary tract endothelial cells on the nanofiber surface was analyzed by calculating the cell adhesion rate and MTT assay, respectively. Cell growth, morphology and distribution on the material surface were observed by fluorescence staining and scanning electron microscopy, respectively.
RESULTS AND CONCLUSION: After 4 weeks of directed differentiation of bone marrow mesenchymal stem cells in vitro, cells showed typical morphology of dendritic bile duct endothelial cells and had the expression of CK19. Scanning electron micrographs showed that electrospun materials were continuous nanofibers with diameters between 200 and 500 nm. No significant degradation of the PLGA nanofibers was observed within 2 weeks. Based on the measured cell adhesion rate, MTT assay, fluorescence staining, and scanning electron microscopy, the differentiated cells possessed a good proliferative capacity on PLGA nanofibers. Bone marrow mesenchymal stem cells differentiated into bile duct endothelial cells in vitro. Materials prepared by the electrospinning method had a nanofiber structure, which did not significantly degrade within 2 weeks. Differentiated cells exhibit good biocompatibility with the nanofibers.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation, Bile Duct Endothelial Cells, Nanofibers, Biocompatible Materials

CLC Number:

R394.2

Yang Yang, Zhou Jia-hua, Yin Xue-yan, Xu Yong, Cao Yang2, Xu Qian. Biocompatibility of porcine bone marrow mesenchymal stem cells-derived bile duct endothelial cells with electrospun nanofibers[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3736-3743.

Figures/Tables 5

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