Biocompatibility of a collagen-heparan sulfate scaffold in the porcine brain

doi:10.3969/j.issn.2095-4344.2015.21.015

Abstract

Abstract:

BACKGROUND: The internal structures of the collagen-heparan sulfate scaffold and human nerve are very similar.
OBJECTIVE: To explore the in vivo biocompatibility of collagen-heparin sulfate scaffold.
METHODS: Forty pigs were randomly divided into two groups, 20 in each group: observation group and control group. Medullo-puncture needle was inserted 1.0 cm adjacent to the midline of anterior fontanelle into the subarachnoid space, and then removed gradually. Collagen-heparin sulfate scaffold was implanted into the observation group, and no treatment was given in the control group. Brain tissues were observed under transmission electron microscope, and cell apoptosis and Caspase-3 expression were detected at days 1, 3, 7, 14 and 30 after surgery.
RESULTS AND CONCLUSION: Under the electron microscope, there were some damaged neurons in the observation group with the emergence of demyelination changes in the myelinated nerve fibers; positive
expression of Caspase-3 protein was found at the junction between the brain tissue and scaffold as well as within the scaffold, but no positive expression was found in the surrounding tissue. There was no cell apoptosis within 30 days after surgery except for individual apoptotic neurons both in the observation group and control group. The number of apoptotic cells in the observation group was higher than that in the control group at days 1, 3, 7, 14 days after surgery (P < 0.05), but there was no difference between the two groups at 30 days after surgery (P > 0.05). Caspase-3 protein expression was at a low state in the two groups, but the protein expression of Caspase-3 was higher in the observation group than the control group at days 3 and 7 after surgery (P < 0.05). These findings indicate that the collagen heparin sulfate scaffold has good biocompatibility in the porcine brain.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Heparitin Sulfate;, Materials Testing, Collagen

CLC Number:

R318

Cao Xiong-bin, Dai Jun, Gong Li, Li Xin, Kuang Liang-hong, Liu Ya-fang, Sun Yuan-ping, Shi Yun-qiong1. Biocompatibility of a collagen-heparan sulfate scaffold in the porcine brain [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3361-3365.

Figures/Tables 2

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