Correlation of serum osteopontin and leptin levels with femoral head necrosis

doi:10.3969/j.issn.2095-4344.2015.20.001

Abstract

Abstract:

BACKGROUND: Both osteopontin and leptin are closely linked to bone metabolism, therefore, which may be related to the attack of femoral head necrosis.
OBJECTIVE: To study the correlation of serum osteopontin and leptin in the development of femoral head necrosis.
METHODS: Thirty-one patients with femoral head necrosis (case group, including 11 cases of ARCO II, 10 of ARCO III, 10 of ARCO IV) and 10 healthy adults (control group) were selected as the research objects. Enzyme-linked immunosorbent assay was used to determine the levels of osteopontin and leptin in serum followed by statistical analysis.
RESULTS AND CONCLUSION: In the case group, the serum levels of osteopontin and leptin were significantly higher than those in the control group (P < 0.05). But the serum levels of osteopontin and leptin showed no correlation between the two groups. These findings indicate that the serum levels of osteopontin and leptin are both elevated in patients with femoral head necrosis, which maybe play a role in the pathogenesis of femoral head necrosis, but there is no obvious correlation.

Key words: Femur Head Necrosis;, Osteopontin, Leptin, Metabolism

CLC Number:

R318

Yan Zhi-kun, Zhao Yu-feng, Yang Shan, Li Hui-jie. Correlation of serum osteopontin and leptin levels with femoral head necrosis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(20): 3117-3123.

Figures/Tables 6

References

[1] Popovic V, Duntas LH. Brain somatic cross-talk: ghrelin, leptin and ultimate challengers of obesity. Nutr Neurosci.2005;8(1): 1-5.

[2] Bartell SM, Rayalam S, Ambati S, et al. Central (ICV) leptin injection increases bone formation, bone mineral density, muscle mass, serum IGF-1, and the expression of osteogenic genes in leptin-deficient ob/ob mice. J Bone Miner Res.2011; 26(8):1710-1720.

[3] 陈卫衡,林娜,郭效东,等.ANFH与而浆脂蛋自的相关性研究[J].中国骨伤,2003,16: 69-70.

[4] Lee JS,Lee JS,RobHL, et al.Alterations in the differentiation ability ofmesenchymal stem cells in patientswith nontraumatic osteonecrosis of the femoralhead: comparative analysis according to the riskfactor. JOrthop Res.2006; 24:604-609.

[5] Suh KT,Kmi SW,Roh HL,et al.Decreased osteogenic differentiation ofmesenchymalstem cells in alcohol-induced osteonecrosis. Clin Orthop Relat Res. 2005:220-225.

[6] Nishida K,Yamamoto T,Motomura G,et al.Pitavastatin may reduce risk of steroid-induced osteonecrosis in rabbits:a preliminary histological study. Clin Orthop Relat Res.2008; 466(5):1054-1058.

[7] Hoshikawa Y, Matsuda Y, Suzuki S, et al. Osteopontin may be responsible for pulmonary vascular remodeling. Chest.2005; 128(6 Suppl):621S.

[8] Myers DL, Harmon KJ, Lindner V, et al. Alterations of arterial physiology in osteopontin-null mice. Arterioscler Thromb Vasc Biol.2003;23(6):1021-1028.

[9] Noda M, Tsuji K, Nifuji A. Osteopontin: a topic from the point of bone morphology. Clin Calcium.2003;13(4):464-466.

[10] Senger DR,Pemizzi CA. Cell migration promoted by a Potent GRGDS-containing thrombin-cleavage fragment of osteopontin.Bioehim BioPhysActa.1996;1314(l-2):13-24.

[11] Mont MA, Hung erford DS. Non-tranmatie avascular necrosis of the femoral h-ead. J Bone Joint Surg (Am).1995;77:459.

[12] Denhardt DT,Guo X.Osteo Pontin:a protein with diverse funetions. FASEB J.1993;7(15):1475-1482.

[13] Ross FP,Chappel J,Alvarez JI,et al.Interactions between the bone matrix p-roteins osteopontin and bone sialoprotein and the osteoclast integrin avβ3 po-tentiated bone resorption.J Biol Chem.1993;268:9901-9907.

[14] Uemura T,Liu YK,Kuboki Y.Preliminary communication. MRNA expression of MT1-MMP,MMP-9,cathepsin K,and TRAP in highly enriched osteoclasts cultured on several matrix proteins and ivory surfaces．Biosci Biotechnol Biochem.2000;64(8): 1771-1773.

[15] Ihara H,Denhardt DT,Furuya K,et al.Parathyroid hormone induced bone resorption does not occur in the absence of osteopontin.J Biol Chem.2001; 276(16):13065-13071.

[16] TakandT,YamanotoT,Ohba Y et al. Role of osteopontin in bone remodeling caused by mechanical stress. J Bone Res.1999; 14:839-849.

[17] Jin C,Miyaura C,Ishimi I,et al.Interleukin 1 regulatesthe expression of o-steopontin mRNA by osteoblast.Mol Cell Endocrinol.1990;74:221-228.

[18] Giachelli CM, Steitz S.Osteopontin: a versatile regulator of inflammation and biomineralization.Matrix Biol.2000;19: 615-622.

[19] 王立平,党耕町.骨桥蛋白在骨愈合过程中的基因表达[J].北京医科大学学报,1998,30(4):355-357.

[20] 熊依林,孙金鹏,等. 骨桥蛋白在关节疾病中的表达及其意义.中国矫形外科杂志,2013,21(4):354-358.

[21] Petrow PK, Hummel KM, Schedel J, et al. Expression of osteopontin mess-enger RNA and protein in rheumatoid arthritis. Arthritis Rheum.2000;43:1597-1605.

[22] Gao SG,Li KH,Zeng KB,et al．Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee oste-oarthritis patients.Osteoarthritis Cartilage. 2010;1:82-87．

[23] Hasegawa M,Segawa T,Maeda M,et al．Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis．J Rheumatol.2011;1: 129-134．

[24] Honsawek S,Tanavalee A,Sakdinakiattikoon M,et al. Correlation of plas-ma and synovial fluid osteopontin with disease severity in knee osteoarthritis.Clin Biochem.2009; 9: 808-812．

[25] Wills AK,Black S,Cooper R,et al.Life course body mass index and risk of k-nee osteoarthritis at the age of 53 years: evidence from the 1946 British birthcohort study.Ann Rheum Dis.2012;71(5):655-660.

[26] 刘琼,李向培.骨桥蛋白在类风湿关节炎发病机制中的作用[J]. 中华风湿病学杂志,2007,11(2):109-111.

[27] Pesesse L,SanehezC,HenrotinY.Osteoehondral plate angiogenesis:a new treatm-ent target in osteoarthritis.Joint Bone Spine.2011;78(2):144-149.

[28] 李霞,袁凤来,赵勃清,等.骨桥蛋白在类风湿性关节炎中的作用[J].中国药理学通报,2012;28(2):155-157.

[29] Sutton S，Clutterbuck A，Harris P，et al.The contribution of the synovium，synovial derived inflammatory cytokines and neuropeptides to the pathogenesis of osteoarthritis.Vet J. 2009;179(1):10-24.

[30] 高曙光,徐文硕,曾凯斌,等.膝关节骨关节炎滑液和软骨骨桥蛋白水平与其病变程度的相关性 [J].中华骨科杂志,2010;30(7): 672-676.

[31] Chan BY,Little CB.The interaction of canonical bone morphogenetic protein-and Wnt-signaling pathways may play an important role in regulating cartilage degradation in osteoarthritis.Arthritis Res Ther.2012;14(3):119-128.

[32] 郑柏生,李锋生. 骨关节炎患者骨桥蛋白、IL-1β表达水平及意义[J].临床合理用药,2014,7(8):1-3.

[33] 胡小东,马信文. 骨关节炎软骨细胞中骨桥蛋白的表达调控研究[J]. 海南医学,2014,25(13):1877-1879.

[34] 肖盛世,高曙光,徐迈,等.人膝骨性关节炎滑膜中表达的骨桥蛋白[J].中国组织工程研究, 2013,17(11):2013-2019.

[35] Gao SG,Cheng L,Zeng C,et al.Usefulness of specific OA biomarkers,thrombin-eleaved osteopontin,in the posterior cruciate ligament OA rabbit model.Osteoarthritis Cartilage. 2013;1:144-150.

[36] 刘滨,刘春阳.骨桥蛋白、白细胞介素-1、肿瘤坏死因子-a,在膝骨关节炎患者血清与滑液中的表达及意义[J].实用医技杂志, 2012, 19(11):1137-1138.

[37] Yamaga M,Tsuji K,Miyatake K,et al.Osteopontin level in synovial fluid is a-ssociated with the severity of joint pain and cartilage degradation after ante-rior cruciate ligament rupture. PLoS One.2012;7(11):49014.

[38] Jiang Y,Yao M,Liu Q,et al.OPN gene polymorphisms influence the risk of knee OA and OPN levels in synovial fluid in a Chinese population.Arthritis Res Ther.2013;15(1):3.

[39] Yumoto K,Ishijima M,Rittling SR,et al．Osteopontin deficiency protects joints against destruction in anti-type Ⅱ collagen antibody-induced arthritis in mice.Proc Natl Acad Sci USA. 2002; 7:4556-4561．

[40] Hoshikawa Y, Matsuda Y, Suzuki S, et al. Osteopontin may be responsible for pu-lmonary vascular remodeling.Chest.2005; 128(6 Suppl):621S.

[41] Myers DL, Harmon KJ, Lindner V, et al.Alterations of arterial physiology in o-steopontin-null mice.Arterioscler Thromb Vasc Biol.2003;23(6):1021-1028.

[42] 杨淑莉,韩梅,温进坤.骨桥蛋白与心血管疾病[J].中国动脉硬化杂志,2000,8(4):371-373.

[43] 张娜,刘蕴玲.骨桥蛋白与动脉粥样硬化和骨质疏松的关系.中国骨质疏松杂志,2012,18(10):949-952.

[44] Hultg rdh-Nilsson A, L vdahl C, Blomgren K, et al. Expression of phenotype- and proliferation-related genes in rat aortic smooth muscle cells in primary culture. Cardiovasc Res.1997; 34(2):418-430.

[45] 傅国香,朱鼎良,刘建军,等.骨桥蛋白参与血管紧张素Ⅱ诱导的血管平滑肌细胞迁移[J]. 高血压杂志, 2006,14(4):277-280.

[46] Zohar R, Suzuki N, Suzuki K, et al. Intracellular osteopontin is an integral component of the CD44-ERM complex involved in cell migration.J Cell Physiol. 2000;184(1):118-130.

[47] Li GH,Oparil S,Kelpke SS,et al.Fibroblast growth factor receptor-1 signal-ing induces osteopontin expression and vascular smooth muscle cell-dependent a-dventitial fibroblast migration in vitro.Circulation.2002;106(7):854-859.

[48] Roy S,Ruest PJ,Hanks SK.FAK regulates tyrosine phosphorylation of CAS,paxillin,and PYK2 in cells expressing v-Src,but is not a critical determinant of v-Src transformation.J Cell Biochem.2002;84(2):377-388.

[49] Yokosaki Y,Matsuura N,SasakiT,et al.The integrin alpha(9)beta(1) binds to a novel recognition sequence (SVVYGLR) in the thrombin-cleaved amino-terminal fragment of osteopontin.J Biol Chem.1999;274(51):36328-36334.

[50] Xie B,Zhao J,Kitagawa M,et al.Focal adhesion kinase activates Stat1 in integrin 2 mediated cell migration and adhesion.J Biol Chem.2001;276 (22):19512-19523.

[51] Tuck AB,Hota C,Wilson SM,et al.Osteopontin-induced migration of human mam-mary epithelial cells involves activation of EGFreceptor and multiple signal t-ransduction pathways.Oncogene.2003;22(8):1198-1205.

[52] 陈晓红,黄海燕,林杨．老年人骨质疏松与动脉硬化关系的初步探讨[J]．福建医药杂志,2009,31(6):4-6．

[53] Burguera B, Hofhauer LC, Thomas T, et al．Leptin reduces ovariectomyinduced bone loss in rats．Endocrinology.2001; 142:3546-3553.

[54] Comish J, Callon KE, Bava U, et al．Leptin directly regulates bone cell function in vitro and reduces bone fragility in vivo. Endocrinol.2002;175:405-415.

[55] Maor G, Rochwerger M, Segev Y, et al. Leptin acts as a growth factor onthe chondrocytes of skeletal growth centers. Bone Miner Res.2002;17:1034-1043．

[56] Reseland JE, Syversen U, Bakke I, et al．Leptin is expressed in and secreted from primary cultures of human osteoblasts and promotes bone mineralization.Bone Minet Res.2001;16: 1426-1433.

[57] Reid IR.Relationships among body mass, its components, and bone.Bone.2002;31(5):547-555.

[58] Whitfield JE. How to grow bone to treat osteoporosis and mend fractures.Curr Rheumatol.2003;5(1):45.

[59] 汪雷,韦永中,曹晓建.等.瘦素对骨代谢影响研究进展[J].国际骨科学杂志,2006;27(6):337-339.

[60] Ducy P,Amling M,Takeda S,et al.Leptin inhibits bone formation through a hyperthalamic relay:a central control of bone mass.Cell.2000;100:197-207.

[61] Reseland JE, Syversen U, Bakke I, et al. Leptin is expressed in and secreted from primary cultures of human osteoblasts and promotes bone mineralization.Bone Miner Res.2001; 16(8):1426-1433.

[62] Gordeladze JO, Drevon CA, Syversen U, et al. Leptin stimulates human osteoblastic cell proliferation, de novo collagen synthesis, and mineralization: Impact on differentiation markers, apoptosis, and osteoclastic signaling. Cell Biochem.2002;85(4):825-836.

[63] Burguera B, Hofbauer LC, Thomas T, et al. Leptin reduces ovariectomy-induced bone loss in rats. Endocrinology.2001; 142(8):3546- 3553.

[64] Hotloway WR,Collier FM,Aitken CJ,et al.Leptin inhibits osteoclast generation.Bone Miner Res.2002;17:200-209.

[65] Gabay C,Dreyer M,Pellegrinelli N,et al.Leptin directly induces the secretion of interleukin 1 receptor antagonist in human monocytes.J Clin EndocrinoI Metab.2001;86:783-789.

[66] Jones JP. Intravascular coagulation and osteonesis. Clin Orthop.1992;277:42.

[67] Jacobs B. Alcohollism induced bone necrosis. NY St ate J Med.1992;92:334.

[68] Glueck CJ, Freiberg RA, Sieve L,et al.Enoxaparin prevents progression of stages Ⅰand Ⅱ osteonecrosis of the hip.Clin Orthop Relat Res.2005;(435):164-170.

[69] Suh KT,Kim SW,Roh HL,et al.Decreased osteogenic differentiation of mesenchymal stem cells in alcohol-induced osteonecrosis.Clin Orthop Relat Res.2005；(431):220-225.

[70] 卢中道,王爱国,王义生.非创伤性股骨头坏死血脂变化临床研究[J].中医正骨,2005,17(10):28.

[71] Meunier PI. Bone histomorphometry in corticosteroid- inducedosteo-porosis and cushing(s)syndronce.Advan Exp Med Bio.1989;171:191.

[72] 洪加源,郭林新,康两期,等.激素性股骨头坏死骨代谢变化的实验研究[J]. 中国骨与关节损伤杂志, 2001,16(5):365-368.

[73] D'Ambrosia RD, Shoji H, Riggins RS, et al. Scintigraphy in the diagnosis of osteonecrosis. Clin Orthop Relat Res.1978;(130): 139-143.

[74] 王辉,李会杰,郑越,等.非创伤性股骨头坏死患者血清瘦素与血脂水平正相关[J].中国组织工程研究与临床康复, 2012,16(52): 9860-9866.