Urine metabonomics of moxibustion products in rats

doi:10.3969/j.issn.2095-4344.2015.15.018

Abstract

Abstract:

BACKGROUND: Moxibustion products produced in moxibustion, such as moxa smoke, are one of hotspots in moxibustion research. Metabonomics can be used to more comprehensively and systematically study the effects of moxibustion products on the body.
OBJECTIVE: To investigate the effect of different concentrations of moxibustion products on urine metabonomics of rats.
METHODS: Forty Sprague-Dawley rats were randomly divided into normal control group, low-dose moxibustion products group, middle-dose moxibustion products group, high-dose moxibustion products group, high-dose moxibustion products recovery group. In the latter four groups, rats from each group were exposed to the mixture of moxibustion products and pure gas at ratios of 0.4:2.0, 0.8:2.0, 1.6:2.0, 1.6:2.0, respectively, 4 hours daily, 5 days weekly, totally for 60 days. After 60-day high-dose moxibustion products stimulation, rats in the high-dose moxibustion products recovery group were raised in normal air for 21 days. Rats in the normal control group were raised in normal air for 60 days without any moxibustion products. Then we analyzed the changes of urine metabonomics in all group rats.
RESULTS AND CONCLUSION: Totally 108 metabolites were identified in the urine of rats using gas chromatography/time-of-flight mass spectrometry, and 64 metabolites were verified by standard library. There were some positive correlations between changes of typical metabolites and moxibustion product concentrations. The metabolites in the urine were most different between the high-dose moxibustion products group and normal control group. Twenty-two differential metabolites, such as glucuronic acid and vitamin C were mainly involved in 15 sugar and amino acid metabolic pathways, such as ascorbate and aldarate metabolism. These findings indicated that energy metabolism, detoxification and anoxidation increased in rats stimulated by moxibustion products.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Urine, Smoke, Metabolism

CLC Number:

R318

Zhou Ci-li, Lu Yuan, Wu Lu-yi, Liu Hui-rong, Cui Yun-hua, Zhao Ji-meng, Li Jing, Zhou Zhi-gang, Wu Huan-gan . Urine metabonomics of moxibustion products in rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(15): 2387-2393.

Figures/Tables 7

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