Mesenchymal stem cells derived from adipose and nucleus pulposus tissue differentiate towards nucleus pulposus-like cells induced by transforming growth factor-beta 1

doi:10.3969/j.issn.2095-4344.2015.10.020

Abstract

Abstract:

BACKGROUND: A large number of studies have shown that adult stem cells derived from multiple tissues are available to differentiate towards nucleus pulposus-like cells in vitro. It is unclear whether mesenchymal stem cells derived from nucleus pulposus tissues have the ability to differentiate towards nucleus pulposus-like phenotypes induced by transforming growth factor-beta 1. Up to now, there are few reports on the difference between the differentiation ability of mesenchymal stem cells derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cells.

OBJECTIVE: To compare the ability of mesenchymal stem cells derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cells differentiating into nucleus pulposus-like cells under induction of transforming growth factor-beta 1.

METHODS: The groin fat tissue and the coccygeal spine of rats were taken respectively to isolate and culture mesenchymal stem cells derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cells by mechanical enzyme digestion method. Flow cytometry was employed to detect the expression of CD105, CD90, CD29, CD45, CD44, CD34, and CD24 of both two kinds of stem cells. Mesenchymal stem cells derived from nucleus pulposus tissues and adipose-derived mesenchymal stem cells were divided into complete induction group (complete induction medium with transforming growth factor-beta 1), incomplete induction group (complete induction medium without transforming growth factor-beta 1) and control group(DMEM/F12 containing 10% fetal bovine serum and 100 mg/L penicillin/streptomycin), respectively. After 14 days of culture, real-time PCR was used to detect the expression of collagen type II, Aggrecan and SOX-9 in each group.

RESULTS AND CONCLUSION: CD105, CD90, CD29 expressed positively and CD45, CD44, CD34, CD24 negatively in both two kinds of stem cells. After 14 days of induced differentiation, the expressions of collagen type II, Aggrecan and SOX-9 in the two kinds of cells were significantly higher in the complete induction groups than in the control groups (P < 0.05). Under the induction of transforming growth factor-beta 1, the expression of collagen type II, Aggrecan and SOX-9 in mesenchymal stem cells derived from nucleus pulposus tissues was significantly higher than that in adipose-derived mesenchymal stem cells (P < 0.05). These findings suggest that both two kinds of mesenchymal stem cells have the ability to differentiate towards nucleus pulposus-like cells induced by transforming growth factor-beta, and mesenchymal stem cells derived from nucleus pulposus tissues may be more suitable as seed cells for nucleus pulposus tissue engineering research.

1库：

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Adipose Tissue, Intervertebral Disk, Mesenchymal Stem Cells, Transforming Growth Factor beta1

CLC Number:

R394.2

Xue Chen-hui, Ma Xun, Guan Xiao-ming, Zhang Hui, Zhang Li. Mesenchymal stem cells derived from adipose and nucleus pulposus tissue differentiate towards nucleus pulposus-like cells induced by transforming growth factor-beta 1[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1585-1891.

Figures/Tables 4

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