Effects of various doses of Liushen Pill on microvessel density in models of esophageal cancer xenografts

doi:10.3969/j.issn.2095-4344.2015.05.019

Abstract

Abstract:

BACKGROUND: Liushen Pill is a traditional Chinese herbal formula, has the effects of heat-clearing and detoxicating, eliminating stagnation, detumescence and alleviating pain. Modern pharmacology verifies that Liushen Pill has anti-inflammatory, analgesic, cardiac, anti-viral, anti-tumor effects, and has been extensively used in the treatment of various infectious diseases and malignant cancer.
OBJECTIVE: To investigate the inhibitory effects of Liushen Pill on esophageal cancer xenografts, and effects on microvessel density and vascular endothelial growth factor expression.
METHODS: After reproducing nude mouse models of human esophageal cancer, 48 nude mice were randomly divided into high-dose Liushen Pill group, moderate-dose Liushen Pill group, low-dose Liushen Pill group, cisplatin group, model group and blank group. According to medication regimen, drugs were given. The growth of transplanted tumor of nude mice was dynamically observed in each group. The nude mice were sacrificed after 20 days of treatment. Neoplasm weight was taken and the tumor-suppressing rate was calculated. Immunohistochemistry was used to detect microvessel density and the expression of vascular endothelial growth factor.
RESULTS AND CONCLUSION: The weight of transplanted tumor was significantly lower in the high-dose Liushen Pill group, moderate-dose Liushen Pill group, low-dose Liushen Pill group, and cisplatin group than in the model group (P < 0.05). Microvessel density and the expression of vascular endothelial growth factor were obviously lower in the each Liushen Pill group than in the model group, but not as apparent as that in the cisplatin group. Results suggested that Liushen Pill can inhibit the growth of the esophageal cancer xenografts. Liushen Pill can down-regulate the expression of vascular endothelial growth factor and reduce microvessel density, which is one of the tumor-inhibiting mechanism of Liushen Pill.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Esophageal Neoplasms, Vascular Endothelial Growth Factors, Drugs, Chinese Herbal

CLC Number:

R318

Zhang Hui, Huang Li-zhong, Tian Sha, Yang Ju, Dai Xin-jun. Effects of various doses of Liushen Pill on microvessel density in models of esophageal cancer xenografts[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 766-771.

Figures/Tables 7

References

[1] 肖国红.六神丸治疗急性化脓性扁桃腺炎的临床疗效观察[J].内蒙古中医药,2013,32(17):26-27.
[2] 李伟,王莉,侯寿安. 六神丸治疗智齿冠周炎的临床研究[J].临床口腔医学杂志,2003,19(5):294.
[3] 贾小靖.六神丸治疗带状疱疹22例疗效分析[J].临床医药实践, 2013,22(5):391-392.
[4] Guan B, Li H, Yang Z,et al.Inhibition of farnesoid X receptor controls esophageal cancer cell growth in vitro and in nude mouse xenografts.Cancer. 2013;119(7):1321-1329.
[5] Moens S, Goveia J, Stapor PC,et al.The multifaceted activity of VEGF in angiogenesis - Implications for therapy responses.Cytokine Growth Factor Rev. 2014 Jul 23. [Epub ahead of print]
[6] Jiang JT, Zhang LF, Zhou B,et al.Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis.Asian Pac J Cancer Prev. 2012;13(7):3379-3383.
[7] Toi M, Matsumoto T, Bando H.Vascular endothelial growth factor: its prognostic, predictive, and therapeutic implications. Lancet Oncol. 2001;2(11):667-673.
[8] Xu XL, Ling ZQ, Chen W,et al.The overexpression of VEGF in esophageal cancer is associated with a more advanced TMN stage: a meta-analysis.Cancer Biomark. 2013;13(2):105-113.
[9] 方立峰,陈宏超,梁倩萍.细胞色素p450 2E1和血管内皮生长因子在食管鳞癌中的表达及临床意义[J].医药论坛杂志,2008,29(16): 6-8.
[10] 曹洋,施瑞华,朱宏.食管鳞癌组织中CTGF和VEGF的表达及其临床意义[J].南京医科大学学报:自然科学版,2008,28(9):1146- 1148.
[11] Su CM, Su YH, Chiu CF,et al.Vascular Endothelial Growth Factor-C Upregulates Cortactin and Promotes Metastasis of Esophageal Squamous Cell Carcinoma.Ann Surg Oncol. 2014 Sep 12. [Epub ahead of print]
[12] Peng J, Shao N, Peng H, et al.Prognostic significance of vascular endothelial growth factor expression in esophageal carcinoma: a meta-analysis.J BUON. 2013; 18(2):398-406.
[13] Cheng AS, Chan HL, To KF,et al.Cyclooxygenase-2 pathway correlates with vascular endothelial growth factor expression and tumor angiogenesis in hepatitis B virus-associated hepatocellular carcinoma.Int J Oncol. 2004;24(4):853-860.
[14] Hong YM, Gan WG, Xu ZH.Significance of the expression of integrin β1, VEGF and MVD in hypopharyngeal squamous cell carcinoma.Genet Mol Res. 2014;13(3):6455-6465.
[15] Triratanachat S, Niruthisard S, Trivijitsilp P,et al.Angiogenesis in cervical intraepithelial neoplasia and early-staged uterine cervical squamous cell carcinoma: clinical significance.Int J Gynecol Cancer. 2006;16(2):575-580.
[16] Bremnes RM, Camps C, Sirera R.Angiogenesis in non-small cell lung cancer: the prognostic impact of neoangiogenesis and the cytokines VEGF and bFGF in tumours and blood. Lung Cancer. 2006;51(2):143-158.
[17] 陶识博,张蕾,张云汉,等. 食管鳞癌中MTAl、MMP-9和MVD的表达及其临床病理意义[J].河南大学学报:医学版,2010,29(1): 29-33.
[18] 齐元富,郑祎,侯倩倩.六神丸以毒攻毒治疗非小细胞肺癌临床研究[J].辽宁中医杂志,2013,40(1):6-7.
[19] 高国青.三七粉冲服六神丸治疗晚期食道癌咽下困难32例[J].陕西中医, 2011,32(9):1123-1124.
[20] 张顺湧.食道癌的中医治疗体会[J].中国实用乡村医生杂志,2009, 16(6):43.
[21] 齐元富,李慧杰,李静.六神丸对H22肝癌腹水移植瘤PDGF与VEGF表达的影响及相关机制探讨[J].世界中医药,2013,8(1): 69-71.
[22] 丁诗语,孙莉,田项楠,等.六神丸对H22肝癌抑瘤及减毒效果的实验研究[J].实用医药杂志,2005,22(7):619-620.
[23] 张春荣,姜伟,齐元富.六神丸对鼠S180生长的抑制作用与抑制血管生成的关系[J].中国预防医学杂志,2005,6(4):327-330.
[24] 刘丹,祝林,奉建芳.蟾酥中蟾毒配基类成分的分离纯化及其体外抗肿瘤活性的研究[J]中成药,2010,32(6):937-940.
[25] 李宗云,高慧敏,王金华.蟾酥总蟾蜍甾烯对H22荷瘤小鼠的抗肿瘤作用及组织中代谢物的初步分析[J]中国中药杂志,2011, 36(21):2987-2993.
[26] 席晓霞,范临兰,田永刚.纳米雄黄的抗小鼠原位乳腺癌作用及其机制[J].中国临床药理学与治疗学,2013,18(9):981-987.
[27] 张亚兰,罗燕,刘春兰,等.中药麝香抗肿瘤研究进展[J].畜牧与饲料科学,2009,30(4):191-192.
[28] 吴志远,李芳芳,张昆.人工牛黄对小鼠乳腺癌肺转移的影响[J]中草药,2012,43(10):2013-2016.