Transplantation of adipose-derived stem cells combined with collagen bioengineering scaffold upregulates vascular endothelial growth factor expression in rats with chronic refractory wound

doi:10.3969/j.issn.2095-4344.0660

Abstract

Abstract:

BACKGROUND: Bioengineering scaffolds are commonly used in the treatment of chronic refractory wounds and have achieved some effects, but there are also some problems. Adipose-derived stem cells (ADSCs) as a new method for the treatment of chronic refractory wounds have its unique efficacy and advantages. However, the efficacy and mechanism of ADSCs combined with bioengineering scaffolds in the treatment of chronic refractory wounds are not yet clear.
OBJECTIVE: To study the effect of ADSCs combined with autologous collagen biomaterial scaffold transplantation on the expression of vascular endothelial growth factor (VEGF) during the healing of chronic refractory wounds.
METHODS: Thirty-one Sprague-Dawley rats were purchased from Shanghai Slack Laboratory Animals Co., Ltd. Adipose tissues from the groin of a rat were harvested, and the primary ADSCs were isolated and cultured in vitro. The remaining 30 rats were randomly divided into normal control group, model group, collagen biomaterial scaffold group (Plenac group), autologous ADSCs transplantation group (ADSCs group) and autologous ADSCs + collagen biomaterial scaffold group (ADSCs+Plenac group), 6 rats in each group. In the normal control group, full-thickness skin wounds in the central and upper part of the bilateral lumbar vertebrae were made, followed by daily routine dressing. In the other groups two chronic refractory wounds were made in the central and upper part of the bilateral lumbar vertebrae followed by local injection of hydrocortisone acetate. Model group were given daily routine treatment; Plenac group treated with fibrin scaffold to cover the wound, followed by daily routine dressing; ADSCs group treated with autologous ADSCs transplantation followed by daily routine dressing; ADSCs+Plenac group received autologous ADSCs transplantation and bioengineering scaffold implantation to cover the wound, followed by daily routine dressing. After 7 days of intervention, all the rats were executed. The following procedures were subsequently carried out: observing wound situation, measuring wound size, detecting the expression of VEGF by immunohistochemical method, detecting the expression of VEGF protein using western blot assay, and detecting the expression of VEGF mRNA by qPCR.
RESULTS AND CONCLUSION: (1) The wound area in the control group was smaller than that in the other groups (P < 0.05), while the wound area in the ADSCs+Plenac group was also smaller than that in the model, Plenac and ADSCs groups (P < 0.05). (2) The expression of VEGF at protein and mRNA levels was higher in the control group compared with the other groups (P < 0.05), while the protein and mRNA expression of VEGF in the ADSCs+Plenac group was significantly higher than that in the model, Plenac and ADSCs group (P < 0.05). To conclude, ADSCs combined with the collagen bioengineering scaffold can upregulate VEGF expression in wound healing, and promote healing of wound healing.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cell Transplantation, Vascular Endothelial Growth Factors, Wound Healing, Tissue Engineering

CLC Number:

R394.2

Zhuang Jing, Yang Yu, Ding Li, Zheng Qing-jian. Transplantation of adipose-derived stem cells combined with collagen bioengineering scaffold upregulates vascular endothelial growth factor expression in rats with chronic refractory wound[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5274-5280.

Figures/Tables 3

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