Expression of matrix structural proteins in the vessel wall of rat models during the early aneurysm formation

doi:10.3969/j.issn.2095-4344.2015.05.016

Abstract

Abstract:

BACKGROUND: Matrix protein is an essential component of the vascular wall, provides a necessary frame for the integrity of the vessel wall and physiological function of vascular wall cells, and regulates cells and smooth muscle.
OBJECTIVE: To construct rat model of early aneurysm, and to evaluate differences in the expression of matrix structural proteins during cerebral aneurysm formation.
METHODS: Twenty-eight healthy male Sprague-Dawley rats were randomized into control group (n=8) and model group (n=20). Aneurysm model was established by ligation of the left common carotid artery and right renal artery-induced hypertension in the model group. In the control group, only the left carotid artery bifurcation and bilateral carotid were exposed in rats. Rats in the model group were sacrificed at 15 and 30 days after model establishment. Right anterior cerebral artery in rats and olfactory artery bifurcation received immunohistochemical staining. The expressions of fibronectin, α-smooth muscle actin and collagen III were analyzed.
RESULTS AND CONCLUSION: Compared with the control group, no significant difference in fibronectin expression was detected in right anterior cerebral artery and olfactory artery bifurcation in rats of the model group at 30 days after model establishment (P > 0.05). However, α-smooth muscle actin and collagen III expressions were significantly reduced (P < 0.05). These data confirmed that expression of structural proteins had differences and dynamic changes during early aneurysm formation in rats. Degradation of matrix structural protein in cerebral artery may be one of the key mechanism of aneurysm formation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Aneurysm, Intracranial Aneurysm, Connexins, Actins, Collagen Type III

CLC Number:

R318

Wang Zeng-liang, Li Shao-shan, Sailike Duishanbai, Wang Yong-xin, Cheng Xiao-jiang, Zhou Qing-jiu, Zhou Kai, Du Guo-jia, Wang Xin, Geng Dangmurenjiafu. Expression of matrix structural proteins in the vessel wall of rat models during the early aneurysm formation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 745-751.

Figures/Tables 5

2.3 脑动脉瘤模型大鼠动脉瘤形成部位中相关基质蛋白的表达 2.3.1 纤维连接蛋白纤维连接蛋白在正常血管壁和动脉瘤形成动脉诱导15 d时血管壁上有表达，其中在1个正常脑动脉标本中染色强，另外6个中较明显染色，而在诱导动脉瘤形成15 d时，10个脑动脉标本中7个中染色清晰，另外3个中染色弱。到30 d时在检测的10个脑动脉标本中表达阳性率为100%，2个标本中染色弱，8个标本中呈染色清晰，模型组大鼠脑动脉标本中纤维连接蛋白平均染色级别与对照组相比差异无显著性意义(P > 0.05；图3，表1)。 2.3.2 α-平滑肌肌动蛋白在检测的8个正常标本中α-平滑肌肌动蛋白呈全阳性，其中在6个正常脑动脉标本中呈强染色，另外2个中为染色清晰，而在诱导动脉瘤形成15 d时的10个脑动脉标本中8个中染色清晰，另外2个中染色弱，到30 d时在检测的10个脑动脉标本中α-平滑肌肌动蛋白的阳性表达率为100%，在7个标本中染色弱，3个标本中染色清晰，模型组大鼠脑动脉标本中α-平滑肌肌动蛋白平均染色级别与对照组相比差异有显著性意义(P=0.005；图4，表1)。 2.3.3 Ⅲ型胶原蛋白在检测的8个正常标本和诱导动脉瘤形成第15天时的10个脑动脉标本中Ⅲ型胶原蛋白呈阳性，主要分布于外膜。其中在5个正常脑动脉标本中染色强，另外3个中为染色清晰，而在诱导动脉瘤形成第15天时的10个脑动脉标本中8个中染色清晰，另外2个染色弱。到诱导动脉瘤形成30 d时在检测的10个脑动脉标本中Ⅲ型胶原蛋白的阳性表达率为100%，在7个标本中染色清晰，3个标本中染色弱。模型组大鼠脑动脉标本中Ⅲ型胶原蛋白平均染色级别与对照组相比差异有显著性意义(P < 0.05；图5，表1)。"

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