Effects of paeoniflorin on the proliferation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.01.018

Abstract

Abstract:

BACKGROUND: Studies have shown that paeoniflorin functions as replenishing blood and treatment of autoimmune diseases, and bone marrow mesenchymal stem cells also play an important role in the body’s blood and immune function. However, paeoniflorin effects on bone marrow mesenchymal stem cell proliferation and cytokine secretion and expression are rarely reported.
OBJECTIVE: To investigate the effect of paeoniflorin on proliferation of bone marrow mesenchymal stem cells and the expression of interleukin-6.
METHODS: Human bone marrow mesenchymal stem cells were separated and cultured in vitro by density gradient centrifugation combined with attachment method. The biological characteristics of bone marrow mesenchymal stem cells were identified by flow cytometry and osteogenic/adipogenic induction. The proliferation of bone marrow mesenchymal stem cells under different concentrations of paeoniflorin was detected by MTT method. The mRNA expression and secretion of interleukin-6 in the supernatant of bone marrow mesenchymal stem cells were detected by RT-PCR and ELISA, respectively.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were isolated successfully and had osteogenic and adipogenic differentiation potential. Compared with the control group, 2 μmol/L and 10 μmol/L paeoniflorin significantly promoted the proliferation of bone marrow mesenchymal stem cells. 10 μmol/L paeoniflorin could significantly decrease the proportion of bone marrow mesenchymal stem cells in G0/G1 phase and increase this proportion in S phase. Compared with the control group, the experimental group could significantly increase the secretion and mRNA expression of interleukin-6 (P < 0.01). It is concluded that paeoniflorin at certain concentrations can obviously promote the proliferation of bone marrow mesenchymal stem cells, and increase the expression and secretion of interleukin-6.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Cell Proliferation, Interleukin-6

CLC Number:

R394.2

Chao Er-tao, Bai Hai, Wang Cun-bang, Xi Rui, Ou Jian-feng, Zhao Qiang. Effects of paeoniflorin on the proliferation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 101-107.

Figures/Tables 7

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