Age-associated proliferation and differentiation changes of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.01.004

Abstract

Abstract:

BACKGROUND: Autologous bone marrow mesenchymal stem cell transplantation for the treatment of cardiovascular disease has become one of the hotspots, but it is unclear whether the proliferation and directional differentiation of autologous bone marrow mesenchymal stem cells varies changes with age.
OBJECTIVE: To explore the proliferation and differentiation changes of rat bone mesenchymal stem cells in different ages.
METHODS: The bone marrow mesenchymal stem cells from Sprague-Dawley rats in different age groups were purified and cultured, and then examined by flow cytometry in terms of cell cycle. Meanwhile, neonatal rat cardiomyocytes were co-cultured with bone marrow mesenchymal stem cells. The morphologic changes of bone marrow mesenchymal stem cells and the protein expression of troponin T were detected with immunofluorescence technique.
RESULTS AND CONCLUSION: The percentage of bone marrow mesenchymal stem cells in G₀/G₁ phase was increased with age; while the percentage of expression of troponin T proteins-positive bone marrow mesenchymal stem cells were decreased with age. These findings indicate that the proliferation and differentiation abilities of rat bone marrow mesenchymal stem cells descend with age.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Troponin T, Cell Proliferation

CLC Number:

R394.2

Li Hai-rui, Zheng Dong, Jiang Can, Guo Jun, Zhang Ai-dong, Li Zi-cheng. Age-associated proliferation and differentiation changes of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 18-23.

Figures/Tables 6

References

[1] Ma Z, Liu Q, Liu H,et al.Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip.Cell Mol Bioeng. 2012;5(3):327-336.
[2] Ma Z, Liu Q, Liu H,et al.Laser-patterned stem-cell bridges in a cardiac muscle model for on-chip electrical conductivity analyses. Lab Chip. 2012;12(3):566-573.
[3] Peters NS, Wit AL.Gap junction remodeling in infarction: does it play a role in arrhythmogenesis.J Cardiovasc Electrophysiol. 2000;11(4):488-490.
[4] Qi BD, Meng BX, Yang Y,et al.Study of immuno-tolerance mechanism of the third-party bone marrow-derived mesenchymal stem cells in allogenic transplantation. Zhonghua Zheng Xing Wai Ke Za Zhi. 2011;27(3):207-212.
[5] Wang Y, Liu J, Xu C,et al.Bone marrow transplantation combined with mesenchymal stem cells induces immune tolerance without cytotoxic conditioning.J Surg Res. 2011; 171(1):e123-131.
[6] Pan H, Zhao K, Wang L,et al.Mesenchymal stem cells enhance the induction of mixed chimerism and tolerance to rat hind-limb allografts after bone marrow transplantation.J Surg Res. 2010;160(2):315-324.
[7] Pittenger MF, Mackay AM, Beck SC,et al.Multilineage potential of adult human mesenchymal stem cells.Science. 1999;284(5411):143-147.
[8] Qayyum AA, Mathiasen AB, Kastrup J.Stem cell therapy to treat heart ischaemia: implications for diabetes cardiovascular complications.Curr Diab Rep. 2014;14(12):554.
[9] Yamawaki-Ogata A, Hashizume R, Fu XM, et al. Mesenchymal stem cells for treatment of aortic aneurysms. World J Stem Cells. 2014;6(3):278-287.
[10] Liu B, Duan CY, Luo CF,et al.Effectiveness and safety of selected bone marrow stem cells on left ventricular function in patients with acute myocardial infarction: A meta-analysis of randomized controlled trials.Int J Cardiol. 2014;177(3): 764-770.
[11] Soleimani M, Nadri S.A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow.Nat Protoc. 2009;4(1):102-106.
[12] Peng L, Jia Z, Yin X, et al.Comparative analysis of mesenchymal stem cells from bone marrow, cartilage, and adipose tissue.Stem Cells Dev. 2008;17(4):761-773.
[13] Wang C, Xu Y, Song WG, et al.Isolation and culturation, phenotype detection of rat bone marrow mesenchymal stem cells.Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(5): 466-468.
[14] Ehler E, Moore-Morris T, Lange S.Isolation and culture of neonatal mouse cardiomyocytes.J Vis Exp. 2013;(79): e50154.
[15] Song YH, Pinkernell K, Alt E.Stem cell induced cardiac regeneration: fusion/mitochondrial exchange and/or transdifferentiation?Cell Cycle. 2011;10(14):2281-2286.
[16] Makkar RR, Price MJ, Lill M,et al. Intramyocardial injection of allogenic bone marrow-derived mesenchymal stem cells without immunosuppression preserves cardiac function in a porcine model of myocardial infarction.J Cardiovasc Pharmacol Ther. 2005;10(4):225-233.
[17] Yin N, Lu R, Lin J,et al.Islet-1 promotes the cardiac-specific differentiation of mesenchymal stem cells through the regulation of histone acetylation.Int J Mol Med. 2014;33(5): 1075-1082.
[18] Hou J, Lü AL, Liu BW,et al.Combination of BMP-2 and 5-AZA is advantageous in rat bone marrow-derived mesenchymal stem cells differentiation into cardiomyocytes.Cell Biol Int. 2013;37(12):1291-1299.
[19] Cheng Z, Ou L, Zhou X,et al.Targeted migration of mesenchymal stem cells modified with CXCR4 gene to infarcted myocardium improves cardiac performance.Mol Ther. 2008;16(3):571-579.
[20] Cheng Z, Liu X, Ou L,et al.Mobilization of mesenchymal stem cells by granulocyte colony-stimulating factor in rats with acute myocardial infarction.Cardiovasc Drugs Ther. 2008; 22(5):363-371.
[21] Ripa RS, Haack-Sørensen M, Wang Y, et al. Bone marrow derived mesenchymal cell mobilization by granulocyte-colony stimulating factor after acute myocardial infarction: results from the Stem Cells in Myocardial Infarction (STEMMI) trial.Circulation. 2007;116(11 Suppl):I24-30.
[22] Rangappa S, Entwistle JW, Wechsler AS,et al. Cardiomyocyte-mediated contact programs human mesenchymal stem cells to express cardiogenic phenotype.J Thorac Cardiovasc Surg. 2003;126(1):124-132.
[23] Fukuhara S, Tomita S, Yamashiro S,et al.Direct cell-cell interaction of cardiomyocytes is key for bone marrow stromal cells to go into cardiac lineage in vitro.J Thorac Cardiovasc Surg. 2003;125(6):1470-1480.
[24] Qu Z, Xu H, Tian Y,et al.Atorvastatin improves microenvironment to enhance the beneficial effects of BMSCs therapy in a rabbit model of acute myocardial infarction.Cell Physiol Biochem. 2013;32(2):380-389.
[25] Li X, Yu X, Lin Q,et al.Bone marrow mesenchymal stem cells differentiate into functional cardiac phenotypes by cardiac microenvironment.J Mol Cell Cardiol. 2007;42(2):295-303.
[26] 郭军,贾光宏,林国生,等.体外模拟心肌微环境下骨髓间充质干细胞向心肌样细胞分化的实验研究[J].心脏杂志,2004,16(6): 501-504.
[27] Stenderup K, Rosada C, Justesen J,et al.Aged human bone marrow stromal cells maintaining bone forming capacity in vivo evaluated using an improved method of visualization. Biogerontology. 2004;5(2):107-118.
[28] Mueller SM, Glowacki J.Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges.J Cell Biochem. 2001; 82(4):583-590.
[29] Zaim M, Karaman S, Cetin G, et al.Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells.Ann Hematol. 2012;91(8): 1175-1186.
[30] Wu J, Niu J, Li X,et al.TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production.BMC Dev Biol. 2014;14:21.
[31] Lu Y, Liu JJ, Bi XY,et al.Pyridostigmine ameliorates cardiac remodeling induced by myocardial infarction via inhibition of the transforming growth factor-β1/TGF-β1-activated kinase pathway.J Cardiovasc Pharmacol. 2014;63(5):412-420.
[32] Bischoff DS, Makhijani NS, Yamaguchi DT.Constitutive expression of human telomerase enhances the proliferation potential of human mesenchymal stem cells.Biores Open Access. 2012;1(6):273-279.
[33] Madonna R, Taylor DA, Geng YJ,et al.Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.Circ Res. 2013;113(7):902-914.
[34] Madonna R, Willerson JT, Geng YJ.Myocardin a enhances telomerase activities in adipose tissue mesenchymal cells and embryonic stem cells undergoing cardiovascular myogenic differentiation.Stem Cells. 2008;26(1):202-211.
[35] Terada N, Hamazaki T, Oka M,et al.Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature. 2002;416(6880):542-545.
[36] Haneef K, Naeem N, Khan I,et al.Conditioned medium enhances the fusion capability of rat bone marrow mesenchymal stem cells and cardiomyocytes.Mol Biol Rep. 2014;41(5):3099-3112.
[37] Ying QL, Nichols J, Evans EP,et al.Changing potency by spontaneous fusion.Nature. 2002;416(6880):545-548.