Thyroxine effects on induced differentiation of human umbilical cord blood-derived mesenchymal stem cells into chondrocytes

doi:10.3969/j.issn.2095-4344.2015.01.002

Abstract

Abstract:

BACKGROUND: Human umbilical cord blood-derived mesenchymal stem cells can be induced through the co-culture to differentiate into other cells, but how to get more seed cells for tissue engineering is one of the most difficult problems.
OBJECTIVE: To investigate the role of the different concentrations of thyroxine in chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells by co-culture with rabbit chondrocytes.
METHODS: Human umbilical cord blood-derived mesenchymal stem cells were co-cultured with rabbit chondrocytes at 2:1, and stimulated by medium containing different concentrations of thyroxine (0, 0.01, 0.1 and 1, 10 μmol/L). Co-cultured cells with no thyroxine served as control group. After 14 days of co-culture, the cell RNA and protein were extracted, mRNA expressions of aggrecan and collagen type II were detected by real-time PCR, and protein expression of aggrecan and collagen type II were detected by western blot assay.
RESULTS AND CONCLUSION: After intervention with 0.01, 0.1, 1, 10 μmol/L thyroxine, the mRNA and protein expressions of aggrecan and collagen type II were enhanced with the increase of thyroxine concentration, which were significantly different from those in the control group (P < 0.05). Experimental findings indicate that high levels of thyroxine can enhance the chondrogenic ability of human umbilical cord blood-derived mesenchymal stem cells co-cultured with rabbit chondrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Fetal Blood, Mesenchymal Stem Cells, Chondrocytes, Coculture Techniques, Thyroxine

CLC Number:

R394.2

Yang Tao, Jiang Bo, Xu Peng, Lin Gang. Thyroxine effects on induced differentiation of human umbilical cord blood-derived mesenchymal stem cells into chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 7-11.

Figures/Tables 3

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