Effect of mouse nerve growth factor on osteoporosis after peripheral nerve injury

doi:10.3969/j.issn.2095-4344.2014.51.007

Abstract

Abstract:

BACKGROUND: Osteoporosis after nerve injury has different pathogenesis from osteoporosis of other causes. A more complex set of factors can be involved in the occurrence and development of osteoporosis after nerve injury. In addition to the loss of stress stimulation, cytokines abnormalities, abnormal nerve function and variation of hormone levels are all involved in osteoporosis after nerve injury.

OBJECTIVE: To observe the interaction between nerve injury and osteoporosis by using living animal experiments.

METHODS: Male Sprague-Dawley rats were randomly divided into normal control and denervation groups. Denervated rat models were caused by cutting off the femoral nerve, and then subdivided into model group and nerve growth factor group. Rats in the nerve growth factor group were subjected to 0.2 mL nerve growth factor injection into the gastrocnemius of bilateral lower limbs, once a day; while rats in the model group were subjected to the same volume of saline. No treatment was done in the normal control group. After 30 days, the rats were weighted and blood samples were collected followed by killing, and then the femur bone was taken out for histomorphometry examination.

RESULTS AND CONCLUSION: After injection of nerve growth factor, the body mass and trabecular bone volume of rats in the nerve growth factor group was significantly higher than those in the model group, indicating local injection of nerve growth factor could obviously reduce the extent of osteoporosis in denervated rats, and meanwhile weaken the weight loss due to denervation. However, there was no change in blood calcium and phosphorus concentrations in each group. Experimental findings suggest that neurological osteoporosis does not originate from the loss of calcium and phosphorus, but by structural changes in the trabecular bone; meanwhile, exogenous mouse nerve growth factor is of positive significance for osteoporosis following after nerve paralysis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: denervation, osteoporosis, nerve growth factor

CLC Number:

R318

Zi Ying, Liu Xin-wei, Li Zhe, Deng Lian-fu. Effect of mouse nerve growth factor on osteoporosis after peripheral nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8233-8237.

Figures/Tables 2

References

[1]Morley J, Marsh S, Drakoulakis E.Does traumatic brain injury result in accelerated fracture healing. Injury.2005; 36(3): 363-368.
[2]王小健,苏云星,余建平,等.感觉/运动神经切断影响大鼠骨折愈合的实验研究[J].中国矫形外科杂志,2008,16(6):450-454.
[3]Otfinowski J. Heterotopic induction of osteogenesis in the couse of neural injury. Patol Pol.1993;44(1):133-168.
[4]Offley SC, Guo TZ, Wei T. Capsaicin-sensitive sensory neurons contribute to the maintenance of trabecular bone integrity. J Bone Miner Res. 2005;20(2):257-267.
[5]Eflefteriou F.Regulation of bone remodeling by the central and peripheral nervous system. Arch Biochem Biophys. 2008;473(2):231-236.
[6]Osen CJ.Bone remodeling, energy metabolism, and the molecular clock. Cell Metab. 2008;7(1):7-10.
[7]Saadoun S, Bell BA, Verkman AS, et al.Greatly improved neurological outcome after spinal cord compression injury in AQP4-deficient mice.Brain.2008;131(4):1087-1098.
[8]Yamaguchi M, Fukagawa M.Role of zinc in regulation ofprotein tyrosine phosphatase activity in osteoblastic MC3T3-E1 cells: zinc modulation of insulin-like growth factor-I's effect. Calcif Tissue Int.2005;76(1):32-38.
[9]Liang D, Yang M, Guo B, et al.Zinc Upregulates the Expression of Osteoprotegerin in Mouse Osteoblasts MC3T3-E1 Through PKC/MAPK Pathways.Biol Trace Elem Res.2012;146:340-348.
[10]Guo B, Yang M, Liang D,et al.Cell apoptosis induced by zinc deficiency in osteoblastic MC3T3-E1cells via a mitochondrial- mediated pathway.Mol Cell Biochem. 2012; 361(1-2):209-216.
[11]Alexandrino AP, Rodrigues MA, Matsuo T,et al.Evaluation of seminal zinc levels by atomic absorption in men with spinal cord injury.Spinal Cord.2011;49(3):435-438.
[12]Wang Y, Me X, Zhang L,et al.Supplement moderate zinc as an effective treatment for spinal cord injury. Med Hypotheses. 2011;77(4):589-590.
[13]陈毅军,赵小纲.神经损伤影响骨折愈合速度机制研究的进展[J].创伤外科杂志,2008,10(1):79-81.
[14]朱建平,张伯勋,刘郑生,等.选择性神经根损伤对大鼠骨代谢影响的实验研究[J].中国矫形外科杂志,2002,9(4):354-356.
[15]Gautschi OP, Toffoli AM, Joesbury KA, et al.Osteoinductive effect of cerebrospinal fluid from brain-injured patients. Neurotrauma. 2007;24(1):154-162.
[16]王玉国,张丹丹.鼠神经生长因子治疗视神经挫伤的研究[J].航空航天医药,2010,21(4):435-436．
[17]杨惠婷,姚鹏,李静秋.视神经钝挫伤的诊治及疗效观察[J].牡丹江医学院学报,2012,33(5):50-52.
[18]张丹丹.鼠神经生长因子结合葛根素对视神经挫伤治疗效果的研究[D].哈尔滨:黑龙江中医药大学,2010.
[19]Lazo MG, Shirazi P, Sam M, et al. Osteoporosis and risk of fracture in men with spinal cord injury. Spinal Cord. 2001;39(4):208-214.
[20]Zhao L, Lv GM, Jiang SY, et al,Donglin Jiang. Morphological differences in skeletal muscle atrophy of rats with motor nerve and/or sensory nerve injury. Neural Regen Res. 2012; 7(32): 2507-2515.
[21]Modlesky CM, Majumdar S, Narasimhan A et al. Trabecular bone microarchit ecture is deteriorated in men with spinal cord injury. J Bone Miner Res.2004;19 (1):48-55.
[22]Vestergaard P, Krogh K, Rejnmark L, et al. Fracture rates and risk factors for fractures in patients with spinal cord injury. Spinal Cord.1998; 36(11): 790-796.
[23]沈凌飞,胡美君.复方樟柳碱联合鼠神经生长因子治疗视神经挫伤疗效分析[J].求医问药:下半月,2011,9(2): 73-74.
[24]陈迎月,郑帆,陈勤.复方樟柳碱联合鼠神经生长因子治疗视神经挫伤的临床观察[J].海峡药学,2011,23(9):102-103.
[25]龚善初,邱久军,李东,等.注射用鼠神经生长因子治疗儿童孤独症的疗效观察[J].中国药业,2012,21 (22): 40-42.
[26]张旭,杨丽娟,陆金梅.鼠神经生长因子治疗视神经萎缩的临床观察[J].中国临床研究,2010,23(6):463-464.
[27]魏智慧,高海雷.腰麻硬膜外联合麻醉致脊髓损伤一例反思[J].临床误诊误治,2010,23(1):79.
[28]高建平,王健,张来虎.胸腰椎骨折合并脊髓损伤的早期手术治疗[J].中国当代医药,2012,19( 33):190-192.
[29]王印国,韦国强,陈娟.鼠神经生长因子治疗急性一氧化碳中毒迟发性脑病临床观察[J].华北国防医药,2010,22(3):227.
[30]Garland DE, Adkins RH, Stewart CA, et al. Regional osteoporosis in women who have a complete spinal cord injury. J Bone Joint Surg Am.2001;83(8):1195-1200.
[31]Warden SJ, Bennell KL, Matthews B, et al.Quantitative ultrasound assessment of acute bone loss following spinal cord injury: a longitudinal pilot study. Osteoporos Int.2002; 13(7):586-592.
[32]Savignat M, De-Doncker L, Vodouhe C, et al. Rat nerve
regeneration with the use of a polymeric membrane loaded with NGF.J Dent Res.2007;86(11):1051-1056.
[33]Amino S, Itakura M, Ohnsi H, et al. Nerve growth factor
enhances neurotransmitterrelease from PC 12 cells by increasing Ca2+-responsible secretory vesicles through the activation of mitogen-activated protein kinase and hosphatidy linositol 3-kinase.J Biochem.2002;131(6):887-894.
[34]Kim HC, Cho YJ, Ahn CW, et al . Nerve growth factor and
expression of itsreceptors inpatients with diabetic europathy.Diabet Med.2009;26(12):1228-1234.
[35]Li XL,Zhang W,Zhou X,et al.Temporal changes in the expression of some neurotrophins in spinal cord transected adult rats.Neuropaptides.2007; 41(3):135－143．
[36]Kasahara K,Nakagawa T,Kubota T.Neuronal loss and expression of neumtrophic factors in a model of rat chronic compressive spinal cord injury.Spine.2006;31(18): 2059-2066.
[37]Sharma HS.A select combination of neurotrophins enhances neu-oprotection and functional recovery following spinal cord injur.Ann N Y A cad Sci. 2007;11(22):95-11．
[38]Swoboda KJ,Kissel JT,Crawford TO,et al.Perspectives on clinical trials in spinal muscular atrophy.J Child Neurol.2007；22(8):957-966．
[39]Wang Y, Zhang H, Wang Z, et al. Therapeutic effect of nerve growth factor on cerebral infarction in dogs using the hemisphere anomalous volume ratio of diffusion-weighted magnetic resonance imaging. Neural Regen Res. 2012; 7(24): 1873-1880.
[40]Sunderland S,Beeadly KC.Stress-strain phenomenia in human peripheral never trunks.Brain.1961;84(2):102．
[41]Yu Y,M atsuyam a Y,Nakashim a S,et al.Effects of M PSS and a potent iNOS inhibitor on traumatic spinal cord injury.Neuroreport.2004;15(13):2103-2107．
[42]Gerndt SJ,Rodriguez L,Pawlik JW,et al. Consequences of high-dose steroid therapy for acute spinal cord injury.J Trauma.1997;42(2):279-284．
[43]Haghighi SS,Agrawal SK,Surdell D Ir,et al.Efects of methylprednisolone and MK-801 on functional recovery after experimental chronic spinal cord injury.Spinal Cord.2000; 38(12):733-740．
[44]Chen ZY,Chai YF,Cao L,et al.G1ial cell line-derived neurotrophic factor enhances axonal regeneration following sciatic nerve transaction in adult rats.Brain Res.2001;902(2)：272-276．
[45]Kasai Y, Iida R, Uchida A. Metal concentrations in the serum and hair of patients with titanium alloy spinal implants. Spine. 2003;28:1320-1326.
[46]Ma SZ, Peng CL, Wu SQ, et al. Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane. Neural Regen Res. 2013; 8(36): 3416-3422.
[47]Closkey RW, Parsons JR,Blacksin MF,et al. Mechanics of interbody spinal fusion:analysis of critical bone graft area. Spine.1993,18:1011.
[48]Adam C,Pearcy M,McCombe P. Stress analysis of interbody fusion--finite element modelling of intervertebral implant and vertebral body. Clin Biomech (Bristol, Avon). 2003;18(4): 265-272.