Isolation and identification of neural stem cells from newborn mouse hippocampus, olfactory bulb and cortex

doi:10.3969/j.issn.2095-4344.2014.45.010

Abstract

Abstract:

BACKGROUND: To in vitro isolate neural stem cells with high purity and uniform biological properties and to establish a complete set of neural stem cell culture system is the basis for neural stem cell research.

OBJECTIVE: To establish an isolation and culture system for neural stem cells from newborn mouse hippocampus, olfactory bulb and cortex and to analyze the biological properties of cells.

METHODS: Neural stem cells were isolated from the hippocampus, olfactory bulb and cortex tissue of newborn Kunming mice by mechanical separation and trypsin digestion. Serum-free culture technology, mechanical pipetting and trypsin digestion were used for subculture of neural stem cells. 10% fetal bovine serum was used to induce differentiation of neural stem cells. Neural stem cells and their differentiated products were identified by immunofluorescent staining of Nestin, CD133, β-TubulinIII, glial fibrillary acidic protein.

RESULTS AND CONCLUSION: The neural stem cell obtained from newborn mouse hippocampus, olfactory bulb and cortex had the capacity of self-renewal and differentiation which were positive for Nestin and CD133. After induction with fetal bovine serum, neural stem cell could differentiation to β-tubulinIII or glial fibrillary acidic protein positive cells that were neurons and astrocytes. This experiment has successfully established the neural stem cell isolation, culture, identification and induction system, providing experimental basis for subsequent studies of neural stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: neural stem cells, mice, hippocampus, olfactory bulb

CLC Number:

R394.2

Ma Jun-ning, Gao Jun-wei, Hou Bo-ru, Ren Hai-jun, Chen Si-hua, Liu Ji-xing, Yan Gui-zhong. Isolation and identification of neural stem cells from newborn mouse hippocampus, olfactory bulb and cortex[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7266-7272.

Figures/Tables 4

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