Differentiation of embryonic stem cells into endothelial progenitor cells under hypoxic condition

doi:10.3969/j.issn.2095-4344.2014.45.008

Abstract

Abstract:

BACKGROUND: The quantity and quality of seed cells is a critical bottleneck of the development of vascular tissue engineering. To address this issue, stem cell-derived endothelial cells have been a hot spot in this field due to their potential in providing the ideal seed cells.

OBJECTIVE: To elucidate the effect of vascular endothelial growth factor (VEGF) supplementation combined with hypoxic culture condition on the lineage-specific differentiation of embryonic stem cells into endothelial cells.

METHODS: Serum-free medium mTeSR^®1 was applied to cultivate H9 cells in vitro. A conditioned medium containing 50 μg/L vascular endothelial growth factor was utilized to induce H9 cells to differentiate into endothelial cells under the hypoxic culture condition (5% O₂). The cell under normal condition (5% CO₂) with or without vascular endothelial growth factor served as controls. The phenotype and function of human embryonic stem cells-derived endothelial cells were assayed by immunofluorescence staining, quantitative RT-PCR, and low-density lipoprotein uptake experiment.

RESULTS AND CONCLUSION: Compared with the control group, the H9 cells were induced to be differentiated into endothelial-like cells more efficiently when they were cultivated under a conditioned medium with vascular endothelial growth factor supplementation under the hypoxic condition. These differentiated cells not only expressed some important surface markers of endothelia cells, including kdr, pecam, but also took in low-density lipoprotein to form microvessle-like structures. This culture system supports a synergy effect of vascular endothelial growth factor and hypoxic environment that can efficiently promotes the lineage-specific differentiation of embryonic stem cells into endothelial cells with good phenotype and functionality.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: embryonic stem cells, endothelial cells, vascular endothelial growth factors, anoxia

CLC Number:

R394.2

Xia Zhen-wei, Wang Ji-wen, Li Xiang-dong, Wei Guo-feng. Differentiation of embryonic stem cells into endothelial progenitor cells under hypoxic condition[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7255-7259.

Figures/Tables 3

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