Optimized medium accelerates differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.45.006

Abstract

Abstract:

BACKGROUND: Studies have found that bone marrow mesenchymal stem cells, under certain conditions, can be induced to differentiate into neurons and glial cells, which to some extent solves the problem of the source of seed cells. Induction methods currently used are different, and their efficiencies are not the same.

OBJECTIVE: To observe the effects of different antioxidants on differentiation of rat bone marrow mesenchymal stem cells into neuron-like cells in vitro.

METHODS: Bone marrow mesenchymal stem cells from Wistar rats were divided into four groups: non-intervention group, β-mercaptoethanol group, retinoic acid group, β-mercaptoethanol+retinoic acid group. Changes in cell morphology and positive rate of neuron-specific enolase and microtubule-associated protein 2 were observed and detected at 5 hours, 12 hours, 1 day, 3 days, 5 days, 7 days, and 10 days after induction.

RESULTS AND CONCLUSION: Except non-intervention group, bone marrow mesenchymal stem cells in the other three groups were gradually becoming spindle-shaped, and gave birth to many small protrusions that were interconnected into a network, showing neuron-like cell morphology. Immunocytochemical staining showed that the efficiency of the β-mercaptoethanol+retinoic acid group was the highest at 10 days after induction, and the positive rates of neuron-specific enolase and microtubule-associated protein 2 were 71.63% and 79.72%, respectively. The results show that β-mercaptoethanol can be combined with retinoic acid to accelerate the differentiation of bone marrow mesenchymal stem cells into neuron-like cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, cell differentiation, tretinoin, mercaptoethanol

CLC Number:

R394.2

Li Qian, Yang Jun. Optimized medium accelerates differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7246-7249.

Figures/Tables 3

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