Effects of three-dimensional spheroid culture system on biological characteristics of mouse bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.45.003

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have attracted widespread attention for the capabilities of self-renewal and muti-differentiation, which have been used in treatment of various diseases.

OBJECTIVE: To study the effect of three-dimensional spheroid culture system on the stemness and senescence of bone marrow mesenchymal stem cells.

METHODS: Mesechyaml stem cells were isolated from the bone marrow of C57/B6 mice, 3 weeks old, and cultured onto the culture plates coated with or without chitosan. After 5 days of culture, the cell phenotype and expression of stemness related markers CD44 and Sca-1 were analyzed by flow cytometry. PI and Annexin-V staining were used to detect cell apoptosis. Also, β-Gal staining was applied for identification of aging.

RESULTS AND CONCLUSION: The mouse mesenchymal stem cells began to form spheroids on day 3. The stemness-related markers, including CD44 and Sca-1, expressed higher in spheroid mesenchymal stem cells than the cells under normal culturing. Compared with the normal culture group, the apoptosis and senescence of cells from spheroid culture were lower. The results indicate that the formation of spheroids on chitosan films can increase the stemness, decrease the apoptosis and slow the senescence of mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, chitosan, apoptosis, cell aging

CLC Number:

R394.2

He Xin, Li Xue, Bao Hui-jing, Wang Ren-feng, Liu Yun-de, Song Shi-wei . Effects of three-dimensional spheroid culture system on biological characteristics of mouse bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7227-7232.

Figures/Tables 6

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