Expansion of chicken bone marrow mesenchymal stem cells by laminin culture system

doi:10.3969/j.issn.2095-4344.2014.45.002

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells from chickens are important cell models for embryonic developmental biology, immunology and oncology research. However, it is difficult to keep bone marrow mesenchymal stem cells with good undifferentiated potential in a large-scale expansion system.

OBJECTIVE: To establish a culture system in vitro with laminin coating to expand bone marrow mesenchymal stem cells from chickens.

METHODS: Isolated bone marrow mesenchymal stem cells from chickens were seeded in laminin-coated plates and traditional two-dimensional plates, respectively. After expansion in vitro, the morphological characteristics, expression of surface markers, expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cells in both conditions were analyzed and compared.

RESULTS AND CONCLUSION: There were no statistical differences in the morphological characteristics and expression of surface markers of bone marrow mesenchymal stem cells expanded by laminin-coated plates and traditional two-dimensional plates. But, the expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cells cultured in laminin-coated plates were better than those in traditional two-dimensional plates. Laminin culture system could quickly amplify out of a large number of chicken bone marrow mesenchymal stem cells with better proliferation ability and undifferentiated performance. All above results indicated that a more efficient expansion system with laminin coating is established.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: mesenchymal stem cells, laminin, extracellular matrix, cell proliferation, chickens

CLC Number:

R394.2

Li Shuang-xing, Qi Yuan, Piao Feng-yuan, Li Ya-chen, Liu Xiao-hui, Shao Jing, Li Shuang-yue. Expansion of chicken bone marrow mesenchymal stem cells by laminin culture system[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7222-7226.

Figures/Tables 4

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