More than 3 ku proteins in chicken egg extract up-regulate expression of pluripotent genes Oct-3/4 and Nanog

doi:10.3969/j.issn.2095-4344.2014.37.023

Abstract

Abstract:

BACKGROUND: Reprogramming somatic cells to generate pluripotent stem cells has a wide application in biomedical research.
OBJECTIVE: To analyze the effect of different molecular weight proteins in chicken egg-white extract to elevate expression of pluripotent genes Oct-3/4 and Nanog in 293T cells.
METHODS: The extracts of chicken egg-white were separated into more than 3 ku and less than 3 ku ingredients to be used for co-culture with 293T cells. There were four groups, 1×10⁵ 293T cells per well, total 500 μL. In the control group, 500 μL culture medium was added; in the other three groups, 500 μL chicken egg-white extract, more than 3 ku and less than 3 ku ingredients were respectively added. Quantitative PCR was used to determine the relative expression levels of pluripotent genes Nanog and Oct-3/4 in 293T cells.
RESULTS AND CONCLUSION: By using co-culture method, more than 3 ku ingredients have a role to increase the expression of pluripotent genes Oct-3/4 and Nanog, but less than 3 ku ingredients cannot elevate the expression of pluripotent genes. This indicates that the ingredient of chicken egg-white extract to elevate the expression of pluripotent genes is more than 3 ku proteins.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: chickens, genes, hek293 cells

CLC Number:

R394.2

Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Shu Fan, Wang Jin-xiang, He Jie, Yang Jian-yong, Zhao Jing, Pang Rong-qing, Pan Xing-hua . More than 3 ku proteins in chicken egg extract up-regulate expression of pluripotent genes Oct-3/4 and Nanog[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 6029-6033.

Figures/Tables 1

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