Molecular ecology of microbial communities in dental plaques of different caries-susceptible children

doi:10.3969/j.issn.2095-4344.2014.25.019

Abstract

Abstract:

BACKGROUND: Children teeth filled with glass ionomer cement are still susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes.
OBJECTIVE: To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children.
METHODS: Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filled index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescent in situ hybridization was used to investigate the numerical level of the caries-induced microbe Streptococcus spp. Quantitative PCR was carried out to analyze relative quantity of Streptococcus mutans in total bacteria.
RESULTS AND CONCLUSION: Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries. Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios of Streptococcus spp. and Streptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can well reflect caries-related complex microbial community in dental plaque.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: mouth, dental plaque, ecology

CLC Number:

R318

Liu Li-xia, Chen Lin. Molecular ecology of microbial communities in dental plaques of different caries-susceptible children[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(25): 4043-4050.

Figures/Tables 3

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